Agriculture and Agri-Food Canada, 107 Science Place, Saskatoon, SK, S7N 0X2, Canada.
Department of Food and Bioproduct Sciences, University of Saskatchewan, Saskatoon, SK, Canada.
Planta. 2022 Oct 6;256(5):93. doi: 10.1007/s00425-022-03998-w.
Genetic variation in seed protein composition, seed protein gene expression and predictions of seed protein physiochemical properties were documented in C. sativa and other Camelina species. Seed protein diversity was examined in six Camelina species (C. hispida, C. laxa, C. microcarpa, C. neglecta, C. rumelica and C. sativa). Differences were observed in seed protein electrophoretic profiles, total seed protein content and amino acid composition between the species. Genes encoding major seed proteins (cruciferins, napins, oleosins and vicilins) were catalogued for C. sativa and RNA-Seq analysis established the expression patterns of these and other genes in developing seed from anthesis through to maturation. Examination of 187 C. sativa accessions revealed limited variation in seed protein electrophoretic profiles, though sufficient to group the majority into classes based on high MW protein profiles corresponding to the cruciferin region. C. sativa possessed four distinct types of cruciferins, named CsCRA, CsCRB, CsCRC and CsCRD, which corresponded to orthologues in Arabidopsis thaliana with members of each type encoded by homeologous genes on the three C. sativa sub-genomes. Total protein content and amino acid composition varied only slightly; however, RNA-Seq analysis revealed that CsCRA and CsCRB genes contributed > 95% of the cruciferin transcripts in most lines, whereas CsCRC genes were the most highly expressed cruciferin genes in others, including the type cultivar DH55. This was confirmed by proteomics analyses. Cruciferin is the most abundant seed protein and contributes the most to functionality. Modelling of the C. sativa cruciferins indicated that each type possesses different physiochemical attributes that were predicted to impart unique functional properties. As such, opportunities exist to create C. sativa cultivars with seed protein profiles tailored to specific technical applications.
在荠蓝和其他荠蓝属物种中记录了种子蛋白组成、种子蛋白基因表达的遗传变异，并预测了种子蛋白的理化性质。在 6 种荠蓝属物种（荠蓝、荠蓝、荠蓝、荠蓝、荠蓝和荠蓝）中检查了种子蛋白多样性。在物种间观察到种子蛋白电泳图谱、总种子蛋白含量和氨基酸组成的差异。为荠蓝属物种编目了编码主要种子蛋白（芸薹素、napins、oleosins 和vicilins）的基因，并通过 RNA-Seq 分析确定了这些基因和其他基因在从开花到成熟的发育种子中的表达模式。对 187 个荠蓝属种质资源的研究表明，种子蛋白电泳图谱的变异有限，但足以根据对应于芸薹素区域的高 MW 蛋白图谱将大多数种质资源分为几类。荠蓝属有四种不同类型的芸薹素，分别命名为 CsCRA、CsCRB、CsCRC 和 CsCRD，它们与拟南芥中的同源物相对应，每种类型的成员都由三个荠蓝属亚基因组上的同源基因编码。总蛋白含量和氨基酸组成仅略有差异；然而，RNA-Seq 分析表明，在大多数品系中，CsCRA 和 CsCRB 基因贡献了芸薹素转录本的>95%，而 CsCRC 基因在其他品系中是最表达的芸薹素基因，包括型品种 DH55。这通过蛋白质组学分析得到了证实。芸薹素是最丰富的种子蛋白，对功能的贡献最大。对荠蓝属芸薹素的建模表明，每种类型都具有不同的理化特性，预计会赋予其独特的功能特性。因此，有机会创建具有特定技术应用所需种子蛋白图谱的荠蓝属品种。
