Li Jinqiu, Wei Xiaofang, Song Yashan, Li Xiaohua, Wang Chengyu
Department of Nephrology, the First Affiliated Hospital of Guangxi Medical University, 6 Shuangyong Road, Nanning, 530021, China.
Curr Microbiol. 2022 Oct 6;79(11):337. doi: 10.1007/s00284-022-03040-y.
In recent years, the occurrence of peritoneal dialysis (PD)-associated peritonitis (PDAP) with Escherichia coli infection has gradually increased. The presence of quorum sensing (QS) among bacteria facilitates the expansion of antibiotic resistance. Brominated furanone (BMF), a halogenated furanone compound isolated from macroalgae, is a new type of quorum-sensing inhibitor that can inhibit bacterial quorum sensing and reduce bacterial resistance. In this study, we established an in vitro peritoneal dialysis-associated peritonitis biofilm model. After intervention with BMF, the biofilm was destroyed, as shown by scanning electron microscopy, and the number of viable bacteria was reduced. Crystal violet semiquantitative determination showed that biofilm absorption significantly decreased, and RT-PCR showed that luxS expression was downregulated after drug intervention. Therefore, we propose that BMF can effectively inhibit E. coli QS by disrupting the bacterial biofilm and downregulating QS gene expression to reduce the bacterial resistance, providing a direction for the development of novel antibacterial drugs.
近年来，由大肠杆菌感染引起的腹膜透析（PD）相关腹膜炎（PDAP）的发生率逐渐上升。细菌群体感应（QS）的存在促进了抗生素耐药性的扩展。溴化呋喃酮（BMF）是一种从大型藻类中分离出的卤代呋喃酮化合物，是一种新型的群体感应抑制剂，可抑制细菌群体感应并降低细菌耐药性。在本研究中，我们建立了体外腹膜透析相关腹膜炎生物膜模型。经BMF干预后，扫描电子显微镜显示生物膜被破坏，活菌数量减少。结晶紫半定量测定显示生物膜吸附显著降低，RT-PCR显示药物干预后luxS表达下调。因此，我们提出BMF可通过破坏细菌生物膜和下调群体感应基因表达来有效抑制大肠杆菌群体感应，从而降低细菌耐药性，为新型抗菌药物的研发提供了方向。
