Cheng Yicheng, Mei Shenglin, Kong Xiangwei, Liu Xianghui, Gao Bo, Chen Bo, Wu Jiang
Department of Stomatology, Jinling Hospital, Medical School of Nanjing University, Nanjing, China.
Department of Prothodontics, College of Stomatology, Xi'an Jiaotong University, Xi'an, China.
J Biomater Appl. 2021 Jan;35(6):643-654. doi: 10.1177/0885328220963934. Epub 2020 Oct 12.
Dental implants are the most innovative and superior treatment modality for tooth replacement. However, titanium implants still suffer from insufficient antibacterial capability and peri-implant diseases remain one of the most common and intractable complications. To prevent peri-implant diseases, a composite coating containing a new antibacterial agent, (Z-)-4-bromo-5-(bromomethylene)-2(5H)-furanone (BBF) was fabricated on titanium. This study was designed to investigate the antibacterial activity of the composite coating against two common peri-implant pathogens ( and ). The morphology of the composite coating showed that BBF-loaded poly(L-lactic acid) nanospheres were well-distributed in the pores of the microarc oxidation coating, and cross-linked with each other and the wall pores by gelatin. A release study indicated that the antibacterial coating could sustain the release of BBF for 60 d, with a slight initial burst release occurring during the first 4 h. The antibacterial rate of the composite coating for adhering bacteria was the highest (over 97%) after 1 d and over 90% throughout a 30-day incubation period. The total fluorescence intensity of the composite coating was the lowest, and the vast majority of the fluorescence was red (dead bacteria). Moreover, real-time polymerase chain reaction analysis confirmed that the relative gene expression of the adherent bacteria on the composite coating was down-regulated. It was therefore concluded that the composite coating fabricated on titanium, which showed excellent and relatively long-term antibacterial activity against and , is a potential and promising strategy to be applied on dental implants for the prevention of peri-implant diseases.
牙种植体是牙齿替代最具创新性和优越性的治疗方式。然而，钛种植体的抗菌能力仍然不足，种植体周围疾病仍是最常见且棘手的并发症之一。为预防种植体周围疾病，在钛表面制备了一种含有新型抗菌剂（Z）-4-溴-5-（溴亚甲基）-2（5H）-呋喃酮（BBF）的复合涂层。本研究旨在探究该复合涂层对两种常见种植体周围病原体（和）的抗菌活性。复合涂层的形态表明，负载BBF的聚（L-乳酸）纳米球均匀分布在微弧氧化涂层的孔隙中，并通过明胶相互交联以及与孔壁交联。释放研究表明，抗菌涂层可使BBF持续释放60天，在最初4小时内有轻微的初始突释。复合涂层对黏附细菌的抗菌率在1天后最高（超过97%），在整个30天的培养期内超过90%。复合涂层的总荧光强度最低，且绝大多数荧光为红色（死菌）。此外，实时聚合酶链反应分析证实，复合涂层上黏附细菌的相对基因表达下调。因此得出结论，在钛表面制备的复合涂层对和具有优异且相对长期的抗菌活性，是一种有潜力且有前景的应用于牙种植体以预防种植体周围疾病的策略。
