Cobb R A, Steer H W
Br J Exp Pathol. 1987 Jun;68(3):461-74.
We describe a new experimental model of mesenteric lymph node metastasis in the rat, involving afferent mesenteric lymphatic inoculation of tumour cell suspensions via glass microcannulae. This model has been used to perform a series of experiments to investigate whether the rat mesenteric lymph node trapped tumour cells. Afferent mesenteric lymphatic inoculation of suspensions of transplantable sarcoma cells in inbred hooded Lister rats resulted in tumour growth in the inoculated lymph node in 100% of rats, with no tumour growth at any other site. The same procedure performed on rats which had previously undergone mesenteric lymphadenectomy resulted in growth of tumour in the lungs. Using 125Iododeoxyuridine (IDUR) labelled sarcoma cells we have shown that although radioactivity decreased significantly in the mesenteric lymph node up to 24 h following afferent lymphatic inoculation, there was no evidence that tumour cells reached thoracic duct lymph. We conclude that the rat mesenteric lymph node trapped sarcoma cells.
我们描述了一种新的大鼠肠系膜淋巴结转移实验模型，该模型通过玻璃微插管将肿瘤细胞悬液接种于肠系膜输入淋巴管。此模型已用于进行一系列实验，以研究大鼠肠系膜淋巴结是否截留肿瘤细胞。将可移植肉瘤细胞悬液接种于近交系带帽利斯特大鼠的肠系膜输入淋巴管，结果100%的大鼠接种淋巴结出现肿瘤生长，其他部位无肿瘤生长。对先前已行肠系膜淋巴结切除术的大鼠进行相同操作，结果肺部出现肿瘤生长。使用125碘脱氧尿苷（IDUR）标记的肉瘤细胞，我们发现，尽管在输入淋巴管接种后长达24小时肠系膜淋巴结中的放射性显著降低，但没有证据表明肿瘤细胞进入胸导管淋巴。我们得出结论，大鼠肠系膜淋巴结截留了肉瘤细胞。
