恶性皮肤肿瘤中自然杀伤细胞密度的体视学研究

NK cell density in malignant skin tumours--a stereological study.

作者信息

Smolle J, Soyer H P, Ehall R, Stettner H, Kerl H

出版信息

Br J Dermatol. 1987 Jun;116(6):823-9. doi: 10.1111/j.1365-2133.1987.tb04901.x.

DOI:10.1111/j.1365-2133.1987.tb04901.x

PMID:3620343

Abstract

We determined the density of natural killer (NK) cells in frozen sections of malignant melanoma (nine cases), squamous cell carcinoma (six cases) and non-neoplastic tonsil (five cases) by immunohistological and stereological methods. The mean density of NK cells in malignant melanoma (5.0 +/- 1.5 X 10(3)/mm3) and in squamous cell carcinoma (5.1 +/- 2.7 X 10(3)/mm3) was significantly lower than in germinal centres of normal human tonsils (5.0 +/- 0.8 X 10(4)/mm3). The NK cell/tumour cell ratio (0.03 +/- 0.015 in malignant melanoma and 0.02 +/- 0.015 in squamous cell carcinoma) is 10(2)-10(4) times lower than that commonly used for in vitro assays. The possible role of NK cells in malignant skin tumours should be viewed with caution.

摘要

我们采用免疫组织学和体视学方法，测定了恶性黑色素瘤（9例）、鳞状细胞癌（6例）及非肿瘤性扁桃体（5例）冰冻切片中自然杀伤（NK）细胞的密度。恶性黑色素瘤（5.0±1.5×10³/mm³）和鳞状细胞癌（5.1±2.7×10³/mm³）中NK细胞的平均密度显著低于正常人类扁桃体生发中心（5.0±0.8×10⁴/mm³）。NK细胞与肿瘤细胞的比例（恶性黑色素瘤中为0.03±0.015，鳞状细胞癌中为0.02±0.015）比体外检测常用比例低10² - 10⁴倍。NK细胞在恶性皮肤肿瘤中的可能作用应谨慎看待。