Bustamante R, Schmitt D, Pillet C, Thivolet J
J Invest Dermatol. 1977 Jun;68(6):346-9. doi: 10.1111/1523-1747.ep12496474.
An immunocytochemical technique has been developed for the identification in situ of immunoglobulin-producing cells in tissues fixed in Bouin's solution and embedded in paraffin. Technical details are discussed as well as the application of the technique to the study of plasma cells in the inflammatory infiltrate around cutaneous tumors. Preliminary results have been obtained with basal cell epitheliomas, squamous cell carcinomas, and malignant melanomas. IgA-producing cells were present in all tumors. IgG-producing cells were present in variable frequency, depending on the type of tumor, and IgM-producing cells were found only in basal cell epitheliomas and squamous cell carcinomas.
已开发出一种免疫细胞化学技术，用于在经布安氏溶液固定并石蜡包埋的组织中原位鉴定产生免疫球蛋白的细胞。文中讨论了技术细节以及该技术在研究皮肤肿瘤周围炎性浸润中浆细胞的应用。对基底细胞上皮瘤、鳞状细胞癌和恶性黑色素瘤已获得初步结果。所有肿瘤中均存在产生IgA的细胞。产生IgG的细胞出现频率各异，取决于肿瘤类型，而产生IgM的细胞仅在基底细胞上皮瘤和鳞状细胞癌中发现。
