上调的芳香烃受体核转位蛋白 2 在鼻咽癌中的潜在分子机制。
Potential Molecular Mechanism of Upregulated Aryl Hydrocarbon Receptor Nuclear Translocator 2 in Nasopharyngeal Carcinoma.
机构信息
Department of Pathology, The First Affiliated Hospital of Guangxi Medical University, No. 6 Shuangyong Road, Nanning, Guangxi Zhuang Autonomous Region 530031, China.
Department of Radiotherapy, Guangxi Medical University Cancer Hospital, No. 71 Hedi Rd, Nanning, Guangxi Zhuang Autonomous Region 530021, China.
出版信息
Comput Math Methods Med. 2022 Sep 27;2022:9137282. doi: 10.1155/2022/9137282. eCollection 2022.
BACKGROUND
Currently, the benefits of nasopharyngeal carcinoma (NPC) therapy are limited, and it is necessary to further explore possible therapeutic targets. Aryl hydrocarbon receptor nuclear translocator 2 (ARNT2) has been extensively studied in other cancer species, but little has been explored in NPC. The aim of this study was to verify the expression level of ARNT2 and its underlying mechanism in NPC.
METHODS
Datasets containing ARNT2 mRNA expression levels were retrieved and collected from various databases to explore the expression status of ARNT2 in NPC. ARNT2-related coexpressed genes, differential expressed genes, and target genes were obtained for functional enrichment analysis. The potential target gene of ARNT2 and their regulatory relationship were studied through ChIP-seq data. CIBERSORTx was used to assess the immune infiltration of NPC, and the association with ARNT2 expression was calculated through correlation analysis.
RESULTS
ARNT2 was upregulated and possessed an excellent discriminatory capability in NPC samples. ARNT2 positively correlated target genes were clustered in pathways in cancer, while negatively correlated target genes were enriched in immune-related pathway. The ChIP-seq information of ARNT2 and histone showed that prostaglandin-endoperoxide synthase 2 (PTGS2) was a potential target gene of ARNT2. CIBERSORTx revealed the immunity status in NPC, and ARNT2 expression was correlated with infiltration of five immune cells.
CONCLUSIONS
ARNT2 is overexpressed in NPC and may regulate PTGS2 to participate in the cancer process. ARNT2 serves as a key oncogenic target in NPC patients.
背景
目前，鼻咽癌（NPC）的治疗效果有限，有必要进一步探索可能的治疗靶点。芳香烃受体核转位蛋白 2（ARNT2）在其他癌症中已经得到了广泛的研究，但在 NPC 中研究甚少。本研究旨在验证 ARNT2 在 NPC 中的表达水平及其潜在机制。
方法
从多个数据库中检索和收集包含 ARNT2 mRNA 表达水平的数据集，以探讨 ARNT2 在 NPC 中的表达状况。获得 ARNT2 相关的共表达基因、差异表达基因和靶基因进行功能富集分析。通过 ChIP-seq 数据研究 ARNT2 的潜在靶基因及其调控关系。使用 CIBERSORTx 评估 NPC 的免疫浸润情况，并通过相关性分析计算与 ARNT2 表达的关联。
结果
ARNT2 在 NPC 样本中上调，具有良好的鉴别能力。ARNT2 的阳性靶基因聚类在癌症途径中，而阴性靶基因富集在免疫相关途径中。ARNT2 和组蛋白的 ChIP-seq 信息表明，前列腺素内过氧化物合酶 2（PTGS2）是 ARNT2 的一个潜在靶基因。CIBERSORTx 揭示了 NPC 中的免疫状态，ARNT2 表达与五种免疫细胞的浸润相关。
结论
ARNT2 在 NPC 中过度表达，可能通过调节 PTGS2 参与癌症进程。ARNT2 是 NPC 患者的关键致癌靶点。
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