Alharbi Khadiga, Alhaithloul Haifa Abdulaziz Sakit, Alayafi Aisha A M, Al-Taisan Wafa'a A, Alghanem Suliman Mohammed, Al-Mushhin Amina A M, Soliman Mona H, Alsubeie Moodi Saham, Vodnar Dan C, Marc Romina Alina
Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia.
Biology Department, College of Science, Jouf University, Sakaka, Saudi Arabia.
Front Plant Sci. 2022 Sep 20;13:999170. doi: 10.3389/fpls.2022.999170. eCollection 2022.
The present study was conducted to examine the potential role of Forsk leaf extract (POLE) which was applied at various concentration levels (control, hydropriming, 10, 20, 30, and 40% POLE) to the wheat ( L.) seedlings. Drought stressed was applied at 60% osmotic potential (OM) to the seedlings to study various parameters such as growth and biomass, photosynthetic pigments and gas exchange characteristics, oxidative stress and response of various antioxidants and nutritional status of the plants. Various growth parameters such as gaseous exchange attributes, antioxidants and nutritional status of were investigated in this study. It was evident that drought-stressed condition had induced a negative impact on plant growth, photosynthetic pigment, gaseous exchange attributes, stomatal properties, and ion uptake by different organs (roots and shoots) of . The decrease in plant growth resulted from oxidative stress and overcome by the antioxidant (enzymatic and non-enzymatic) compounds, since their concentration increased in response to dehydration. Seed priming with POLE positively increased plant growth and photosynthesis, by decreasing oxidative stress indicators and increasing activities of antioxidant (enzymatic and non-enzymatic) compounds, compared to the plants which were grown without the application of POLE. Our results also depicted that optimum concentration of POLE for seedlings under drought condition was 20%, while further increase in POLE (30 and 40%) induced a non-significant ( < 0.05) effect on growth (shoot and root length) and biomass (fresh and dry weight) of seedling. Here we concluded that the understanding of the role of seed priming with POLE in the increment of growth profile, photosynthetic measurements and nutritional status introduces new possibilities for their effective use in drought-stressed condition and provides a promising strategy for tolerance against drought-stressed condition.
本研究旨在考察不同浓度(对照、水引发、10%、20%、30%和40%的POLE)的福斯树叶提取物(POLE)对小麦幼苗的潜在作用。对幼苗施加60%渗透势(OM)的干旱胁迫,以研究各种参数,如生长和生物量、光合色素和气体交换特性、氧化应激以及各种抗氧化剂的反应和植物的营养状况。本研究调查了各种生长参数,如气体交换属性、抗氧化剂和营养状况。显然,干旱胁迫条件对植物生长、光合色素、气体交换属性、气孔特性以及小麦不同器官(根和茎)的离子吸收产生了负面影响。植物生长的下降是由氧化应激引起的,并被抗氧化剂(酶促和非酶促)化合物克服,因为它们的浓度随着脱水而增加。与未施用POLE的植物相比,用POLE进行种子引发通过降低氧化应激指标和增加抗氧化剂(酶促和非酶促)化合物的活性,积极地促进了植物生长和光合作用。我们的结果还表明,干旱条件下小麦幼苗的POLE最佳浓度为20%,而POLE浓度进一步增加(30%和40%)对小麦幼苗的生长(茎和根长度)和生物量(鲜重和干重)产生了不显著(P<0.05)的影响。在此我们得出结论,了解用POLE进行种子引发在生长状况改善、光合测量和营养状况方面的作用,为其在干旱胁迫条件下的有效利用带来了新的可能性,并为小麦耐受干旱胁迫条件提供了一种有前景的策略。