Maidadi Barthelemy, Ntchapda Fidèle, Miaffo David, Kamgue Guessom Oulianovie
Department of Biological Sciences, Faculty of Sciences, University of Maroua, P.O. Box 814, Maroua, Cameroon.
Department of Biological Sciences, Faculty of Science, University of Ngaoundere, P.O. Box 454, Ngaoundere, Cameroon.
Evid Based Complement Alternat Med. 2022 Sep 27;2022:9519743. doi: 10.1155/2022/9519743. eCollection 2022.
(Rubiaceae) is a plant used in African medicine for the treatment of diabetes. The aim of this study was to evaluate the antioxidant, enzyme inhibitory, and hypoglycemic effects of extract (RSE). The contents of phenols, tannins, and flavonoids were determined by phytochemical screening. 2,2-Azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and 2,2-diphenylpicrylhydrazyl (DPPH) were determined to evaluate the free radical scavenging capacity of the RSE. The inhibitory activity of -amylase and -glucosidase was evaluated using the -amylase and -glucosidase inhibition methods and using the sucrose and starch tolerance tests. The glucose tolerance test was performed on normal rats using doses of 50, 100, and 200 mg/kg of RSE. RSE contains total phenols (36.35 mg GAE/g of extract), flavonoids (11.91 mg QE/g of extract), and tannins (13.01 mg CE/g of extract). RSE exhibits significant radical scavenging activity on DPPH and ABTS radicals with an IC of 17.51 and 21.89 g/mL, respectively. RSE showed an inhibitory effect on the activity of -amylase and -glucosidase with an IC of 308.93 and 354.13 g/mL, respectively. RSE (100 and 200 mg/kg) caused a significant decrease in area under the curve and postprandial glycemia at 60, 90, and 120 min following the administration of starch or sucrose. Regarding the glucose tolerance test, RSE (100 and 200 mg/kg) significantly reduced postprandial hyperglycemia from the 90th min posttreatment. RSE lowered postprandial hyperglycemia and has antioxidant properties. These effects would be due to the presence of bioactive compounds in the RSE.
茜草科植物是一种在非洲医学中用于治疗糖尿病的植物。本研究的目的是评估提取物（RSE）的抗氧化、酶抑制和降血糖作用。通过植物化学筛选测定酚类、单宁和黄酮类化合物的含量。采用2,2-联氮-双-3-乙基苯并噻唑啉-6-磺酸（ABTS）和2,2-二苯基-1-苦基肼基（DPPH）法评估RSE的自由基清除能力。采用淀粉酶和葡萄糖苷酶抑制法以及蔗糖和淀粉耐量试验评估淀粉酶和葡萄糖苷酶的抑制活性。使用50、100和200mg/kg的RSE剂量对正常大鼠进行葡萄糖耐量试验。RSE含有总酚（36.35mg没食子酸当量/g提取物）、黄酮类化合物（11.91mg槲皮素当量/g提取物）和单宁（13.01mg儿茶素当量/g提取物）。RSE对DPPH和ABTS自由基具有显著的清除活性，IC50分别为17.51和21.89μg/mL。RSE对淀粉酶和葡萄糖苷酶的活性具有抑制作用，IC50分别为308.93和354.13μg/mL。RSE（100和200mg/kg）在给予淀粉或蔗糖后60、90和120分钟时，显著降低曲线下面积和餐后血糖。关于葡萄糖耐量试验，RSE（100和200mg/kg）从治疗后第90分钟起显著降低餐后高血糖。RSE降低餐后高血糖并具有抗氧化特性。这些作用可能归因于RSE中生物活性化合物的存在。
