Berdan R C, Bulloch A G
Brain Res. 1987 Aug 4;417(1):153-7. doi: 10.1016/0006-8993(87)90191-0.
Exposure of the buccal ganglion of the mollusc, Helisoma trivolvis, to tannic acid ringer (TARI), pH 6.8, permits the ultrastructural detection of exocytotically released material within the neuropil. At pH 4.0, tannic acid penetrated cell membranes and resulted in a marked enhancement in the electron density of regularly speed microtubule associated proteins. Electrophysiological analysis of neurons 19, 4 and 5 exposed to TARI, pH 6.8 and 4.0, demonstrated that the TARI procedure altered the neuronal firing patterns in an irreversible manner. We conclude that the neurotoxic effects of TARI preclude its usefulness in quantitative ultrastructural analysis of exocytotic events, but that at pH 4.0 it is useful for ultrastructural investigations of microtubules and their associated proteins.
将淡水螺（Helisoma trivolvis）的颊神经节暴露于pH值为6.8的鞣酸林格液（TARI）中，可在神经纤维网内通过超微结构检测到胞吐释放的物质。在pH值为4.0时，鞣酸穿透细胞膜，导致正常排列的微管相关蛋白的电子密度显著增加。对暴露于pH值为6.8和4.0的TARI中的神经元19、4和5进行电生理分析表明，TARI处理以不可逆的方式改变了神经元的放电模式。我们得出结论，TARI的神经毒性作用使其无法用于胞吐事件的定量超微结构分析，但在pH值为4.0时，它可用于微管及其相关蛋白的超微结构研究。
