Chalke Saili, Vidovic Sinisa, Fletcher Graham C, Palmer Jon, Flint Steve
Food Safety and Preservation Team, The New Zealand Institute for Plant and Food Research Limited, Auckland, New Zealand.
Institute of Food Science and Technology, School of Food and Advanced Technology, Massey University, Palmerston North, New Zealand.
Biofouling. 2022 Sep;38(8):786-795. doi: 10.1080/08927014.2022.2131398. Epub 2022 Oct 9.
is a gram-positive foodborne pathogen that causes outbreaks of listeriosis associated with a diverse range of foods forms biofilms as a strategy to enhance its survival in the environment. These biofilms then provide a source of contamination in processing plant environments. Cations like magnesium, calcium, and sodium are commonly found in the environment and are important to bacteria to maintain their homeostasis. It is, therefore, valuable to understand the relationship between these cations and biofilm formation. In this study, four isolates of from seafood processing environments were used to investigate the influence of magnesium, calcium, and sodium (1, 10, and 50 mM) on biofilms. The isolates selected were defined as being either a low biofilm former, a high biofilm former, an outbreak isolate, and a persistent isolate from the seafood industry. The study showed that the divalent cations magnesium and calcium increased biofilm formation compared with the monovalent cation, sodium. Fifty mM concentrations of the divalent cations significantly enhanced biofilm formation. The cations did not have a significant effect on the initial stages of biofilm formation but appeared to influence the later stages of biofilm development.
是一种革兰氏阳性食源性病原体，可引发与多种食品相关的李斯特菌病暴发，它形成生物膜作为增强其在环境中生存能力的一种策略。这些生物膜随后成为加工厂环境中的污染源。镁、钙和钠等阳离子在环境中普遍存在，对细菌维持其体内平衡很重要。因此，了解这些阳离子与生物膜形成之间的关系很有价值。在本研究中，使用从海鲜加工环境中分离出的四株菌株来研究镁、钙和钠（1、10和50 mM）对生物膜的影响。所选的分离株被定义为低生物膜形成菌、高生物膜形成菌、暴发分离株和来自海鲜行业的持续存在分离株。研究表明，与单价阳离子钠相比，二价阳离子镁和钙增加了生物膜的形成。50 mM浓度的二价阳离子显著增强了生物膜的形成。这些阳离子对生物膜形成的初始阶段没有显著影响，但似乎影响生物膜发育的后期阶段。
