Department of Orthopaedics, The Second Affiliated Hospital of Air Force Military Medical University, Xi'an, China.
Department of Orthopaedics, The First Affiliated Hospital of Harbin Medical University, Harbin, China.
Front Immunol. 2022 Sep 21;13:987344. doi: 10.3389/fimmu.2022.987344. eCollection 2022.
Spinal cord injury refers to damage to the spinal cord due to trauma, disease, or degeneration; and the number of new cases is increasing yearly. Significant cellular changes are known to occur in the area of spinal cord injury. However, changes in cellular composition, trajectory of cell development, and intercellular communication in the injured area remain unclear. Here, we used single-cell RNA sequencing to evaluate almost all the cell types that constitute the site of spinal cord injury in rats. In addition to mapping the cells of the injured area, we screened the expression of immune autophagy-related factors in cells and identified signaling pathways by the measuring the expression of the receptor-ligand pairs to regulate specific cell interactions during autophagy after spinal cord injury. Our data set is a valuable resource that provides new insights into the pathobiology of spinal cord injury and other traumatic diseases of the central nervous system.
脊髓损伤是指由于创伤、疾病或退化导致的脊髓损伤；且每年新发病例数量都在增加。已知在脊髓损伤区域会发生显著的细胞变化。然而，损伤区域内细胞组成、细胞发育轨迹和细胞间通讯的变化仍不清楚。在这里，我们使用单细胞 RNA 测序来评估构成大鼠脊髓损伤部位的几乎所有细胞类型。除了绘制损伤区域的细胞图谱外，我们还筛选了细胞中免疫自噬相关因子的表达，并通过测量受体-配体对的表达来鉴定信号通路，以调节脊髓损伤后自噬过程中特定细胞间的相互作用。我们的数据集是一个有价值的资源，为脊髓损伤和中枢神经系统其他创伤性疾病的病理生物学提供了新的见解。
