Evensen J F, Sommer S, Moan J, Christensen T
Cancer Res. 1984 Feb;44(2):482-6.
Both 3H-labeled and unlabeled hematoporphyrin derivative (HPD) were analyzed by high-pressure liquid chromatography (HPLC) and gel permeation chromatography. Four main components were isolated by HPLC, and two were isolated by gel permeation chromatography. The tumor-localizing ability of each component was tested and compared to that of 67Ga and 3H2O by injection in mice bearing Lewis lung carcinoma. The photosensitizing abilities of the HPLC-separated components in vitro were also tested. Finally, porphyrin extracts of tumors from mice given HPD were analyzed by HPLC. The tumor-localizing ability of the components increased with decreasing polarity. While crude HPD localized in tumor tissue only to the same extent as did 3H2O, Component 7 was almost as effective as was 67Ga in localizing in the tumor. The cellular uptake of HPD components increased with decreasing polarity. In accordance with this, the low-polarity components were the most effective photosensitizers.
采用高压液相色谱（HPLC）和凝胶渗透色谱法对3H标记和未标记的血卟啉衍生物（HPD）进行分析。通过HPLC分离出4种主要成分，通过凝胶渗透色谱法分离出2种成分。通过给荷Lewis肺癌的小鼠注射，测试了每种成分的肿瘤定位能力，并与67Ga和3H2O的肿瘤定位能力进行比较。还测试了HPLC分离成分在体外的光敏能力。最后，通过HPLC分析给予HPD的小鼠肿瘤的卟啉提取物。成分的肿瘤定位能力随极性降低而增强。虽然粗制HPD在肿瘤组织中的定位程度仅与3H2O相同，但成分7在肿瘤定位方面几乎与67Ga一样有效。HPD成分的细胞摄取随极性降低而增加。据此，低极性成分是最有效的光敏剂。
