Tan Lei, Zhou Yujun, Qiu Yixing, Lei Lei, Wang Cheng, Zhu Pei, Duan Deyong, Lei Hongyu, Yang Lincheng, Wang Naidong, Yang Yi, Yao Jun, Wang Wei, Wang Aibing
Lab of Animal Disease Prevention and Control and Animal Model, Hunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines, College of Veterinary Medicine, Hunan Agricultural University (HUNAU), Changsha, China.
Hunan Sino-science Gene Technology Co., Ltd, Changsha, China.
Front Vet Sci. 2022 Sep 16;9:973450. doi: 10.3389/fvets.2022.973450. eCollection 2022.
Pseudorabies virus (PRV) is widely prevalent in China, which can transmit from pigs to other mammals. Moreover, a PRV variant isolated from an acute human encephalitis case was documented recently. It is imperative to investigate PRV epidemiology in pigs, the knowledge regarding pseudorabies (PR) and self-protection behaviors upon working among relevant practitioners including pig farmers, pig cutters, and pork salesman. In the present study, 18,812 pig serum samples and 1,634 tissue samples were collected from Hunan Province during the period of 2020 to 2021 for detecting the presence of PRV gE-special antibody and nucleic acids, respectively. Meanwhile, we conducted a questionnaire survey about PR among these practitioners in China. The results showed that nearly 9% (1,840/20,192) pigs from 161 collected sites (20.17%, 161/797) were seropositive for PRV-gE antibody. Though only 2.33% tissue samples were positive for PRV nucleic acids, all the representative PRV strains were variant. It was learned that most practitioners were frequently injured when working, the injured sites mainly included hand and foot. Among the three transmission routes of PRV, the aerosol transmission route was often overlooked. Moreover, the workers lacked self-protection awareness and were poor conscious about PRV and its potential threat to humans. All the results demonstrate that PRV remains widely spread in pig populations, while the potential threats of PRV in pig industry receive less attention, suggesting that targeted educational programs to these people should be performed.
伪狂犬病病毒（PRV）在中国广泛流行，可从猪传播给其他哺乳动物。此外，最近有文献记载了从一例急性人类脑炎病例中分离出的PRV变异株。调查猪群中PRV的流行病学情况、了解伪狂犬病（PR）相关知识以及包括养猪户、杀猪工和猪肉销售员在内的相关从业者在工作时的自我保护行为势在必行。在本研究中，于2020年至2021年期间从湖南省采集了18812份猪血清样本和1634份组织样本，分别用于检测PRV gE特异性抗体和核酸的存在情况。同时，我们对中国这些从业者进行了关于PR的问卷调查。结果显示，来自161个采集地点（占797个地点的20.17%，即161/797）的近9%（1840/20192）的猪PRV - gE抗体血清呈阳性。虽然只有2.33%的组织样本PRV核酸呈阳性，但所有代表性PRV毒株均为变异株。据悉，大多数从业者在工作时经常受伤，受伤部位主要包括手和脚。在PRV的三种传播途径中，气溶胶传播途径常常被忽视。此外，这些工人缺乏自我保护意识，对PRV及其对人类的潜在威胁认识不足。所有结果表明，PRV在猪群中仍广泛传播，而PRV在养猪业中的潜在威胁受到的关注较少，这表明应该针对这些人群开展有针对性的教育项目。
