Lin Yuan, Tan Lei, Wang Changjian, He Shicheng, Fang Ling, Wang Zicheng, Zhong Yating, Zhang Kun, Liu Daoxin, Yang Qing, Wang Aibing
Hunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines, College of Veterinary Medicine, Hunan Agricultural University, Changsha, China.
Hunan Provincial Center for Animal Disease Control and Prevention, Changsha, China.
Front Vet Sci. 2021 Dec 16;8:762326. doi: 10.3389/fvets.2021.762326. eCollection 2021.
Pseudorabies (PR), caused by variant pseudorabies virus (PRV), is an economically important viral disease in China. Recently, PRV infection in humans has also received attention worldwide. To investigate the PRV infection in Hunan province, China, we collected a total of 18,138 serum specimens from 808 PRV-vaccinated pig farms cross this region during 2016-2020, and we detected the presence of PRV glycoprotein B (gB) and gE-specific antibodies. The enzyme-linked immunosorbent assay (ELISA) results revealed that 80.47% (14,596/18,138, 95 CI 79.9-81.0) and 23.55% (4,271/18,138, 95 CI 22.9-24.2) of serum samples were positive for PRV gB and gE-specific antibodies, respectively. Further analysis indicated that the seroprevalence of wild PRV infection was associated with the season and breeding scale ( < 0.01). In addition, five PRV strains were isolated from PRV-positive samples in Vero cells and the virus titers varied from 10 to 10 TCID/0.1 ml. The phylogenetic analysis revealed that one isolate was a classical strain of PRV genotype II, and four other isolates belonged to the variants of genotype II. Collectively, the data indicate that the prevalence of PRV remains high in pigs in Hunan province, and the variant PRV strains are the major genotypes affecting the development of the pig industry.
伪狂犬病（PR）由变异伪狂犬病病毒（PRV）引起，是中国一种具有重要经济影响的病毒性疾病。最近，PRV在人类中的感染也受到了全球关注。为调查中国湖南省的PRV感染情况，我们在2016年至2020年期间从该地区808个接种PRV疫苗的猪场共收集了18138份血清样本，并检测了PRV糖蛋白B（gB）和gE特异性抗体的存在情况。酶联免疫吸附测定（ELISA）结果显示，分别有80.47%（14596/18138，95%置信区间79.9 - 81.0）和23.55%（4271/18138，95%置信区间22.9 - 24.2）的血清样本PRV gB和gE特异性抗体呈阳性。进一步分析表明，野生PRV感染的血清阳性率与季节和养殖规模相关（P < 0.01）。此外，从PRV阳性样本中在Vero细胞中分离出5株PRV毒株，病毒滴度在10³至10⁵TCID/0.1 ml之间。系统发育分析表明，一株分离株是PRV基因型II的经典毒株，其他四株分离株属于基因型II的变异株。总体而言，数据表明湖南省猪群中PRV的流行率仍然很高，变异PRV毒株是影响养猪业发展的主要基因型。
