Gomes Ana Carolina, Borges Alexandre, Zoca Daniela Garcia, Silva Marcio Luís Andrade E, Machado Aline Rafaela da Silva Rodrigues, Machado Alex Martins, Santos Mario F C, de Laurentiz Rosangela da Silva
Departamento de Física e Química, Faculdade de Engenharia de Ilha Solteira, UNESP - Univ Estadual Paulista, Ilha Solteira, São Paulo, Brazil.
Faculdade de Medicina, Centro Universitário - UNIFUNEC, Santa Fé do Sul, São Paulo, Brazil.
Nat Prod Res. 2023 Aug-Sep;37(16):2787-2794. doi: 10.1080/14786419.2022.2131784. Epub 2022 Oct 10.
is the primary vector of virus transmission that causes dengue, yellow fever, chikungunya and zika. The primary prevention method has been vector control and synthetic insecticides that can cause environmental side effects. Thus, the work aimed to evaluate the larvicidal potential of extracts and isolated compounds from against larvae. The larvicidal activity method was executed according to the World Health Organization protocol. The larvae were analyzed by scanning electron microscopy (SEM). Through molecular docking, the action mechanism was investigated. The hydroalcoholic and hexane extracts showed similar larvicidal activity with LC of 191.1 μg/mL and 185.84 μg/mL, respectively. Between isolated compounds, hinokinin presented LC= 97.74 μg/mL. The SEM analysis showed structural damage to the larva's tegument caused by extracts and isolated compounds. Therefore, the results demonstrate the larvicidal action of hinokinin and extracts, which can lead to the development of new natural larvicides.
是导致登革热、黄热病、基孔肯雅热和寨卡病毒的主要病毒传播媒介。主要预防方法一直是病媒控制以及会造成环境副作用的合成杀虫剂。因此，这项工作旨在评估 提取物和分离化合物对 幼虫的杀幼虫潜力。杀幼虫活性方法按照世界卫生组织的方案执行。通过扫描电子显微镜（SEM）对幼虫进行分析。通过分子对接研究作用机制。水醇提取物和己烷提取物表现出相似的杀幼虫活性，LC50分别为191.1μg/mL和185.84μg/mL。在分离化合物中，扁柏素的LC50 = 97.74μg/mL。SEM分析显示提取物和分离化合物对幼虫体表造成了结构损伤。因此，结果证明了扁柏素和提取物的杀幼虫作用，这可能会促成新型天然杀幼虫剂的开发。
