Cárdenas-Escudero J, Galán-Madruga D, Cáceres J O
Laser Chemistry Research Group, Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of Madrid, Plaza de Ciencias 1, 28040, Madrid, Spain; Analytical Chemistry Department, FCNET, University of Panama, University City, University Mail, 3366, Panama 4, Panama City, Panama.
National Centre for Environmental Health. Carlos III Health Institute, Ctra. Majadahonda-Pozuelo km 2.2, 28220, Majadahonda, Madrid, Spain.
Talanta. 2023 Feb 1;253:123961. doi: 10.1016/j.talanta.2022.123961. Epub 2022 Sep 24.
The adulteration of honey (Apis mellifera) is a global problem due to its economic, commercial and health implications. The world's leading beekeeping organisation, APIMONDIA, considers that the detection of adulteration in honey is a problem that has not yet been resolved. This evidence of the importance of the intensive development of analytical techniques that allow the unequivocal detection of adulterants in honey, especially those whose use as honey adulterants has recently emerged. This work aims to develop a fast, easy-to-perform, low-cost analytical method to qualitatively and quantitatively determine rice syrup using the Fourier transform infrared spectroscopy (FTIR) technique with attenuated total reflectance (ATR) mode without complex mathematical procedures and sophisticated sample preparation. This study involved the analysis of 256 intentionally rice-syrup-adulterated honey samples and 92 pure honey samples of bee multifloral honey from Spain. The method, based strictly on the determination of the absorbance directly from the samples, at 1013 cm The methodology used no need for previous treatments or preparations and demonstrated the scope for the unequivocal detection of rice syrup in adulterated honey containing equal to or higher than 3% (m/m) or more of this adulterant. Using the Exponential Plus Linear model (r = 0.998) shows high accuracy and precision, in terms of relative error (0.32%, m/m) and coefficient of variation (1.4%). The results of this study have led to the establishment of a maximum absorbance threshold of 0.670 for honey without rice syrup.
由于蜂蜜（意大利蜜蜂）掺假所涉及的经济、商业和健康问题，它已成为一个全球性难题。世界领先的养蜂组织——国际养蜂业委员会（APIMONDIA）认为，蜂蜜掺假的检测仍是一个尚未解决的问题。这表明大力发展分析技术至关重要，这些技术要能明确检测出蜂蜜中的掺假物，尤其是那些近期才出现的用作蜂蜜掺假物的物质。本研究旨在开发一种快速、易于操作且低成本的分析方法，利用傅里叶变换红外光谱（FTIR）技术的衰减全反射（ATR）模式，在无需复杂数学程序和精细样品制备的情况下，定性和定量测定大米糖浆。本研究分析了256个故意掺有大米糖浆的蜂蜜样品以及92个来自西班牙的蜜蜂多花蜂蜜纯蜂蜜样品。该方法严格基于直接测定样品在1013 cm处的吸光度。所使用的方法无需预先处理或制备，并证明了在掺假蜂蜜中明确检测出大米糖浆的范围，该掺假物含量等于或高于3%（m/m）。使用指数加线性模型（r = 0.998），在相对误差（0.32%，m/m）和变异系数（1.4%）方面显示出高精度和高准确度。本研究结果已确定不含大米糖浆的蜂蜜的最大吸光度阈值为0.670。
