Orfanos A P, Guthrie R, Jinks D C
Clin Chim Acta. 1987 Jul 15;166(2-3):219-25. doi: 10.1016/0009-8981(87)90424-4.
We describe a microfluorometric method for determination of carnosinase activity in dried blood spots on filter paper. The carnosinase in discs punched from such dried blood specimens is activated with cadmium at 30 degrees C. After incubation with the substrate carnosine at the same temperature, histidine is determined fluorometrically. We compare the results of this method with those of a fluorometric method using liquid blood and assess the stability of the enzyme in dried blood on filter paper. The presence of erythrocytes has no effect on the activity. The method may be useful in early detection of carnosinase deficiency and certain other metabolic disorders.
我们描述了一种用于测定滤纸上干血斑中肌肽酶活性的微量荧光测定法。从这种干血标本上 punched 出的圆片中的肌肽酶在30摄氏度下用镉激活。在相同温度下与底物肌肽孵育后，用荧光法测定组氨酸。我们将该方法的结果与使用液体血液的荧光法的结果进行比较，并评估滤纸上干血中酶的稳定性。红细胞的存在对活性没有影响。该方法可能有助于早期检测肌肽酶缺乏症和某些其他代谢紊乱。 （注：原文中“punched”此处可能有误，暂按此翻译，也可能是“cut”等词）
