Orfanos A P, Naylor E W, Guthrie R
Clin Chem. 1980 Jul;26(8):1198-200.
We describe a microfluorometric method for determination of arginase activity in dried blood spots on filter paper. The arginase in discs punched from such dried blood specimens is activated by preincubation with Mn2+ at 37 degrees C. After incubation with substrate at the same temperature, urea is determined fluorometrically by oxidation of NADH to NAD+ in a coupled kinetic reaction. We compare the results of this method with those of a colorimetric method involving liquid blood samples, and assess the stability of the enzyme in dried blood on filter paper. The presence of serum has no effect on the activity. This method may be useful in the early detection of arginase deficiency and certain hematological disorders.
我们描述了一种用于测定滤纸干血斑中精氨酸酶活性的微量荧光测定法。从此类干血标本上 punched 出的圆片中的精氨酸酶，通过在37摄氏度下与Mn2+预孵育来激活。在相同温度下与底物孵育后，通过在偶联动力学反应中将NADH氧化为NAD+，用荧光法测定尿素。我们将该方法的结果与涉及液体血样的比色法结果进行比较，并评估滤纸干血中酶的稳定性。血清的存在对活性没有影响。该方法可能有助于精氨酸酶缺乏症和某些血液系统疾病的早期检测。
