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通过葡萄糖转运蛋白 1（GLUT1）作为门控靶点，利用 M1 巨噬细胞选择性探针可视化炎症。

Visualizing inflammation with an M1 macrophage selective probe via GLUT1 as the gating target.

机构信息

School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology (POSTECH), Pohang, 37673, Republic of Korea.

Center for Self-assembly and Complexity, Institute for Basic Science (IBS), Pohang, 37673, Republic of Korea.

出版信息

Nat Commun. 2022 Oct 10;13(1):5974. doi: 10.1038/s41467-022-33526-z.

DOI:10.1038/s41467-022-33526-z
PMID:36216803
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9550770/
Abstract

Macrophages play crucial roles in protecting our bodies from infection and cancers. As macrophages are multi-functional immune cells, they have diverse plastic subsets, such as M1 and M2, derived from naïve M0 cells. Subset-specific macrophage probes are essential for deciphering and monitoring the various activation of macrophages, but developing such probes has been challenging. Here we report a fluorescent probe, CDr17, which is selective for M1 macrophages over M2 or M0. The selective staining mechanism of CDr17 is explicated as Gating-Oriented Live-cell Distinction (GOLD) through overexpressed GLUT1 in M1 macrophages. Finally, we demonstrate the suitability of CDr17 to track M1 macrophages in vivo in a rheumatoid arthritis animal model.

摘要

巨噬细胞在保护我们的身体免受感染和癌症方面发挥着至关重要的作用。由于巨噬细胞是多功能免疫细胞，它们有多种可塑性亚群，如 M1 和 M2，源自幼稚的 M0 细胞。亚群特异性巨噬细胞探针对于解析和监测巨噬细胞的各种激活至关重要，但开发此类探针一直具有挑战性。在这里，我们报告了一种荧光探针 CDr17，它对 M1 巨噬细胞具有选择性，而对 M2 或 M0 巨噬细胞没有选择性。CDr17 的选择性染色机制通过在 M1 巨噬细胞中过表达 GLUT1 被解释为基于门控的活细胞区分（GOLD）。最后，我们证明了 CDr17 适合在类风湿关节炎动物模型中追踪体内的 M1 巨噬细胞。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b242/9550770/157cc56f9c65/41467_2022_33526_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b242/9550770/8f6e26ce0f93/41467_2022_33526_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b242/9550770/641f91aecb7d/41467_2022_33526_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b242/9550770/8847b771a51d/41467_2022_33526_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b242/9550770/157cc56f9c65/41467_2022_33526_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b242/9550770/8f6e26ce0f93/41467_2022_33526_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b242/9550770/641f91aecb7d/41467_2022_33526_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b242/9550770/8847b771a51d/41467_2022_33526_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b242/9550770/157cc56f9c65/41467_2022_33526_Fig4_HTML.jpg

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