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新型水凝胶系统消除了传代培养，并提高了非衰老间充质干细胞群体的保留率。

Novel hydrogel system eliminates subculturing and improves retention of nonsenescent mesenchymal stem cell populations.

机构信息

Bioengineering Graduate Program, University of Kansas, Lawrence, KS 66045, USA.

Department of Plastic Surgery, University of Kansas Medical Center, Kansas City, KS 66160, USA.

出版信息

Regen Med. 2023 Jan;18(1):23-36. doi: 10.2217/rme-2022-0140. Epub 2022 Oct 12.

DOI:10.2217/rme-2022-0140
PMID:36222003
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9732917/
Abstract

To compare the physiological behavior of mesenchymal stem/stromal cells (MSCs) within an expandable tissue-mimetic 3D system relative to expansion in a traditional 2D system. Adipose-derived MSCs (ASCs) were continuously cultured for 6 weeks on either 2D culture plastic or in a 3D hydrogel system that eliminated subculturing. ASCs were assessed for senescence, 'stem-like' MSC markers, and ability for their secretome to augment a secondary cell population. The 3D hydrogel system resulted in an enhanced retention of more regenerative, nonsenescent ASC populations that exhibited increased expression of 'stem-like' MSC surface markers. This study introduces a proof-of-concept design for a novel modular 3D system that can improve expansion of stem-like cell populations for future regenerative therapies.

摘要

为了比较可扩展的组织模拟 3D 系统中间充质干细胞/基质细胞 (MSCs) 的生理行为与传统 2D 系统中的扩展情况。将脂肪来源的间充质干细胞 (ASCs) 连续培养 6 周，分别在 2D 培养塑料或在 3D 水凝胶系统中培养，该系统消除了传代培养。评估 ASCs 的衰老、“干细胞样”MSC 标志物以及其分泌组增强二次细胞群的能力。3D 水凝胶系统导致更具再生性、非衰老的 ASC 群体的保留得到增强，这些群体表现出“干细胞样”MSC 表面标志物的表达增加。这项研究介绍了一种新型模块化 3D 系统的概念验证设计，该系统可用于未来的再生治疗，以改善“干细胞样”细胞群体的扩增。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c913/9732917/abb8a1db484b/rme-18-23-g4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c913/9732917/3eab0e301bd1/rme-18-23-g1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c913/9732917/6e35f15bb031/rme-18-23-g2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c913/9732917/9aa13b87f0d5/rme-18-23-g3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c913/9732917/abb8a1db484b/rme-18-23-g4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c913/9732917/3eab0e301bd1/rme-18-23-g1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c913/9732917/6e35f15bb031/rme-18-23-g2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c913/9732917/9aa13b87f0d5/rme-18-23-g3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c913/9732917/abb8a1db484b/rme-18-23-g4.jpg

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本文引用的文献

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2
Neuronal Differentiation from Induced Pluripotent Stem Cell-Derived Neurospheres by the Application of Oxidized Alginate-Gelatin-Laminin Hydrogels.通过应用氧化海藻酸盐-明胶-层粘连蛋白水凝胶从诱导多能干细胞衍生的神经球诱导神经元分化。
Biomedicines. 2021 Mar 5;9(3):261. doi: 10.3390/biomedicines9030261.
3
Effect of Long-Term 3D Spheroid Culture on WJ-MSC.
用于再生医学的水凝胶工程的进展与挑战
Gels. 2024 Mar 30;10(4):238. doi: 10.3390/gels10040238.
4
Characterization of 3D Organotypic Culture of Mouse Adipose-Derived Stem Cells.鼠脂肪来源干细胞 3D 器官型培养的特性。
Int J Mol Sci. 2024 Apr 1;25(7):3931. doi: 10.3390/ijms25073931.
5
Mesenchymal Stem Cell Extracellular Vesicles from Tissue-Mimetic System Enhance Epidermal Regeneration via Formation of Migratory Cell Sheets.组织模拟系统来源的间充质干细胞细胞外囊泡通过形成迁移性细胞片增强表皮再生。
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6
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7
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World J Stem Cells. 2019 Dec 26;11(12):1065-1083. doi: 10.4252/wjsc.v11.i12.1065.
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