Hall D W, van den Hoven W E
Drugs. 1987;33 Suppl 2:30-40. doi: 10.2165/00003495-198700332-00008.
Using filtration and viscosity techniques, cyclandelate, some of its metabolites and flunarizine were evaluated to determine their ability to maintain human red blood cell (RBC) deformability. In filtration studies, RBC suspensions were metabolically depleted, which decreased the capacity of the cells to pass through narrow filter pores. In viscosity measurements performed with whole blood, metabolic depletion resulted in an increase of blood viscosity values. The results of these experiments appeared to indicate that cyclandelate, cyclandelate alcohol and flunarizine were approximately equipotent in maintaining RBC filtration and blood viscosity values. Cyclandelate acid appeared to be less active in both studies. By measuring the adenosine triphosphate content of red blood cells it has been shown that this activity is not due to an action on red blood cell energy metabolism. Finally, experiments conducted with the calcium chelator EGTA showed that the activity of cyclandelate was reduced in the absence of Ca++ ions. The results strengthen the hypothesis that cyclandelate maintains red cell deformability by inhibiting calcium entry through the red cell membrane.
运用过滤和黏度技术，对环扁桃酯、其部分代谢产物以及氟桂利嗪进行了评估，以确定它们维持人类红细胞（RBC）变形能力的水平。在过滤研究中，红细胞悬液的代谢被耗尽，这降低了细胞通过狭窄滤孔的能力。在用全血进行的黏度测量中，代谢耗尽导致血液黏度值升高。这些实验结果似乎表明，环扁桃酯、环扁桃酯醇和氟桂利嗪在维持红细胞过滤和血液黏度值方面大致具有同等效力。在两项研究中，环扁桃酯酸的活性似乎较低。通过测量红细胞中的三磷酸腺苷含量发现，这种活性并非源于对红细胞能量代谢的作用。最后，用钙螯合剂乙二醇双四乙酸（EGTA）进行的实验表明，在没有钙离子的情况下，环扁桃酯的活性降低。这些结果强化了这样一种假设，即环扁桃酯通过抑制钙离子通过红细胞膜的内流来维持红细胞的变形能力。
