Bianchi S, Brasili F, Saglimbeni F, Cortese B, Di Leonardo R
Opt Express. 2022 Jun 20;30(13):22321-22332. doi: 10.1364/OE.454910.
Optical diffraction tomography (ODT) is a label-free technique for three dimensional imaging of micron-sized objects. Coherence and limited sampling of 3D Fourier space are often responsible for the appearance of artifacts. Here we present an ODT microscope that uses low temporal coherence light and spatial light modulators to retrieve reliable 3D maps of the refractive index. A common-path interferometer, based on a spatial light modulator, measures the complex fields transmitted by a sample. Measured fields, acquired while scanning the illumination direction using a digital micro-mirror device, are fed into a Rytov reconstruction algorithm to obtain refractive index maps whose accuracy is directly evaluated on microfabricated 3D test objects. Even for challenging shapes such as pyramids, bridges, and dumbbells, we obtain volumetric reconstructions that compare very well with electron microscopy images.
光学衍射层析成像（ODT）是一种用于微米级物体三维成像的无标记技术。三维傅里叶空间的相干性和有限采样常常导致伪像的出现。在此，我们展示了一种ODT显微镜，它使用低时间相干光和空间光调制器来获取可靠的折射率三维图谱。一种基于空间光调制器的共光路干涉仪测量样品透射的复场。在使用数字微镜器件扫描照明方向时采集的测量场被输入到Rytov重建算法中，以获得折射率图谱，其准确性在微加工的三维测试物体上得到直接评估。即使对于诸如金字塔、桥梁和哑铃等具有挑战性的形状，我们也能获得与电子显微镜图像非常吻合的体积重建结果。
