Interdisciplinary Program in Bioengineering, Seoul National University, Seoul, Republic of Korea.
Department of Mechanical Engineering, Seoul National University, Seoul, Republic of Korea.
Methods Mol Biol. 2023;2563:383-394. doi: 10.1007/978-1-0716-2663-4_19.
Phase separation is a key mechanism for intracellular organization, driving the segregation of biomolecules into distinct condensates. Intracellular condensates play diverse functional roles including gene expression, stress response, and cell signaling. Technologies that enable the control of intracellular phase separation can be highly useful not only for a better understanding of the biophysical principles of phase separation processes but also for engineering novel condensates. Here, we describe an optogenetic approach for spatiotemporal control of phase separation in living cells.
相分离是细胞内组织的关键机制，它促使生物分子分离成不同的凝聚物。细胞内凝聚物发挥着多种功能作用，包括基因表达、应激反应和细胞信号传递。能够控制细胞内相分离的技术不仅对于更好地理解相分离过程的生物物理原理非常有用，而且对于工程新型凝聚物也非常有用。在这里，我们描述了一种光遗传学方法，用于在活细胞中进行时空控制的相分离。
