Department of Medical Biotechnology and Nanotechnology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Applied Biomedical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Environ Sci Pollut Res Int. 2023 Feb;30(7):19313-19325. doi: 10.1007/s11356-022-23518-3. Epub 2022 Oct 13.
In this study, the manufacture of zinc oxide nanoparticles (ZnO-NPs) was completed via the sol-gel method with Trigonella foenum-graecum L extract for the first time to function as the stabilizing and reducing agent. The obtained product was investigated by various analyzing procedures such as TGA/DTG, FT-IR, UV-Vis, XRD, and EDX/FESEM. The calcination of our product was conducted at temperatures of 400, 500, and 600 °C. In conformity to the XRD pattern, heightening the temperature of calcination caused an enlargement in the size of nanoparticles. The photocatalytic performance of ZnO-NPs was evaluated to degrade methylene blue and Eriochrome black T (EBT) dyes under UV light, which resulted in a degradation percentage of about 96% and 94%, after 90 min, respectively. There has been some evidence suggesting that the green synthesis of ZnO-NPs has increased their use in medicine. The outcomes of examining the cytotoxicity effect of this product against the Huh-7 cell line by the performance of the MTT assay were indicative of an IC of around 62.5 µg/mL. Finally, according to the results of the broth microdilution method, which was performed to assess the antibacterial activity of ZnO-NPs towards gram-positive and gram-negative bacteria, the value of MIC was in the range of 31 to 125 µg/mL. The obtained results from biological studies confirm the antibacterial and anticancer properties of ZnO-NPs, which are promising for applying NPs in medical fields.
在这项研究中，首次通过溶胶-凝胶法用三叶草（Trigonella foenum-graecum L）提取物制造氧化锌纳米粒子（ZnO-NPs），作为稳定剂和还原剂。通过 TGA/DTG、FT-IR、UV-Vis、XRD 和 EDX/FESEM 等各种分析程序研究了所得到的产物。我们的产品在 400、500 和 600°C 的温度下进行煅烧。根据 XRD 图谱，升高煅烧温度会导致纳米粒子的尺寸增大。评估了 ZnO-NPs 的光催化性能，以在紫外光下降解亚甲基蓝和 Eriochrome 黑 T（EBT）染料，分别在 90 分钟后达到约 96%和 94%的降解率。有证据表明，ZnO-NPs 的绿色合成增加了其在医学中的应用。通过 MTT 测定法评估该产品对 Huh-7 细胞系的细胞毒性作用的结果表明，IC 约为 62.5µg/mL。最后，根据肉汤微量稀释法评估 ZnO-NPs 对革兰氏阳性和革兰氏阴性菌的抗菌活性的 MIC 值范围为 31 至 125µg/mL。生物学研究的结果证实了 ZnO-NPs 的抗菌和抗癌特性，这为将 NPs 应用于医学领域提供了前景。
