Tsegahun Elmineh, Aklilu Muluken
Department of Chemistry, College of Science, Bahir Dar University, Bahir Dar City, Ethiopia.
Discov Nano. 2025 Aug 22;20(1):145. doi: 10.1186/s11671-025-04260-4.
In this study, we prepared zinc oxide nanoparticles using a quick, safe, and cost-effective method by reducing Zn(NO)·6HO solution with Neem (Azadirachta indica) leaf extract. Qualitative phytochemical screening and FT-IR spectroscopy measurements were employed to validate the presence of active biomolecules such as Flavonoids, phenols, alkaloids, terpenes and tannic compounds. FT-IR, UV-Vis, and XRD spectroscopic techniques were utilized to fully analyze the biosynthesized nanoparticles. The spectrum of UV-Visible spectroscopy indicated UV-Vis spectrum of 321 nm. FTIR spectra showed the absorption peak for the stretching vibration of Zn-O at 544 cm. The results obtained supported the formation of ZnO NPs employing A. indica leaf extract as a reducing and stabilizing agent. X-ray diffraction spectrum analysis was also used to investigate the crystal structure. The particle size of ZnO NPs was calculated using the Scherrer's equation and the result was found to be 19.16 nm. Furthermore, the antibacterial potential of zinc oxide nanoparticles against two clinical strains of Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) bacteria was examined by paper disc diffusion method. The result showed a significant inhibition zone of 18 mm against E. coli and an inhibition zone of 15 mm against S. aureus.
在本研究中，我们通过用印楝（Azadirachta indica）叶提取物还原Zn(NO)·6HO溶液，采用一种快速、安全且经济高效的方法制备了氧化锌纳米颗粒。采用定性植物化学筛选和傅里叶变换红外光谱（FT-IR）测量来验证黄酮类、酚类、生物碱、萜类和单宁化合物等活性生物分子的存在。利用FT-IR、紫外可见光谱（UV-Vis）和X射线衍射（XRD）光谱技术对生物合成的纳米颗粒进行全面分析。紫外可见光谱显示其光谱在321nm处。傅里叶变换红外光谱显示Zn-O伸缩振动的吸收峰在544cm处。所得结果支持了以印楝叶提取物作为还原剂和稳定剂形成氧化锌纳米颗粒。还使用X射线衍射光谱分析来研究晶体结构。利用谢乐方程计算氧化锌纳米颗粒的粒径，结果为19.16nm。此外，通过纸片扩散法检测了氧化锌纳米颗粒对两种临床菌株大肠杆菌（E. coli）和金黄色葡萄球菌（S. aureus）的抗菌潜力。结果显示对大肠杆菌有18mm的显著抑菌圈，对金黄色葡萄球菌有15mm的抑菌圈。
