Izquierdo Fernando, Ollero Dolores, Magnet Angela, Galván-Díaz Ana L, Llorens Sergio, Vaccaro Lucianna, Hurtado-Marcos Carolina, Valdivieso Elizabeth, Miró Guadalupe, Hernández Leticia, Montoya Ana, Bornay-Llinares Fernando J, Acosta Lucrecia, Fenoy Soledad, Del Águila Carmen
Facultad de Farmacia, Universidad San Pablo-CEU, CEU Universities, Urbanización Montepríncipe, 28660 Boadilla del Monte, Spain.
Grupo de Microbiología Ambiental, Escuela de Microbiología, Universidad de Antioquia, Medellín 050010, Colombia.
Animals (Basel). 2022 Sep 20;12(19):2507. doi: 10.3390/ani12192507.
is one of the world's most endangered felines inhabiting the Iberian Peninsula. The present study was performed to identify the presence of microsporidia due to the mortality increase in lynxes. Samples of urine ( = 124), feces ( = 52), and tissues [spleen ( = 13), brain ( = 9), liver ( = 11), and kidney ( = 10)] from 140 lynxes were studied. The determination of microsporidia was evaluated using Weber's chromotrope stain and Real Time-PCR. Of the lynxes analyzed, stains showed 10.48% and 50% positivity in urine and feces samples, respectively. PCR confirmed that 7.69% and 65.38% belonged to microsporidia species. The imprints of the tissues showed positive results in the spleen (38.46%), brain (22.22%), and liver (27.27%), but negative results in the kidneys. PCR confirmed positive microsporidia results in 61.53%, 55.55%, 45.45%, and 50%, respectively. Seroprevalence against was also studied in 138 serum samples with a positivity of 55.8%. For the first time, the results presented different species of microsporidia in the urine, feces, and tissue samples of . The high titers of anti- antibodies in lynx sera confirmed the presence of microsporidia in the lynx environment. New studies are needed to establish the impact of microsporidia infection on the survival of the Iberian lynx.
是栖息在伊比利亚半岛的世界上最濒危的猫科动物之一。由于猞猁死亡率上升，本研究旨在确定微孢子虫的存在情况。对140只猞猁的尿液样本（n = 124）、粪便样本（n = 52）以及组织样本[脾脏（n = 13）、大脑（n = 9）、肝脏（n = 11）和肾脏（n = 10）]进行了研究。使用韦伯氏变色染色法和实时聚合酶链反应（Real Time-PCR）评估微孢子虫的检测情况。在所分析的猞猁中，染色显示尿液和粪便样本中的阳性率分别为10.48%和50%。聚合酶链反应证实7.69%和65.38%属于微孢子虫物种。组织印记显示脾脏（38.46%）、大脑（22.22%）和肝脏（27.27%）呈阳性结果，但肾脏呈阴性结果。聚合酶链反应分别证实微孢子虫阳性结果在脾脏、大脑、肝脏和肾脏中为61.53%、55.55%、45.45%和50%。还对138份血清样本进行了针对[具体物质未给出]的血清阳性率研究，阳性率为55.8%。首次在猞猁的尿液、粪便和组织样本中发现了不同种类的微孢子虫。猞猁血清中高滴度的抗[具体物质未给出]抗体证实了猞猁环境中存在微孢子虫。需要开展新的研究来确定微孢子虫感染对伊比利亚猞猁生存的影响。
