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加那利群岛海岸(西班牙)商业鱼类中异尖线虫属(线虫纲:异尖科)物种的形态学和分子鉴定:流行病学数据

Morphological and Molecular Identification of spp. (Nematoda: Anisakidae) in Commercial Fish from the Canary Islands Coast (Spain): Epidemiological Data.

作者信息

Martin-Carrillo Natalia, García-Livia Katherine, Baz-González Edgar, Abreu-Acosta Néstor, Dorta-Guerra Roberto, Valladares Basilio, Foronda Pilar

机构信息

Instituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias, Universidad de La Laguna, Avda. Astrofísico F. Sánchez s/n, 38203 San Cristóbal de La Laguna, Canary Islands, Spain.

Department Obstetricia y Ginecología, Pediatría, Medicina Preventiva y Salud Pública, Toxicología, Medicina Legal y Forense y Parasitología, Universidad de La Laguna, Avda. Astrofísico F. Sánchez s/n, 38203 San Cristóbal de La Laguna, Canary Islands, Spain.

出版信息

Animals (Basel). 2022 Sep 30;12(19):2634. doi: 10.3390/ani12192634.

DOI:10.3390/ani12192634
PMID:36230375
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9559264/
Abstract

The study aimed to perform the molecular identification of larvae in commercial fish from the coast of the Canary Islands and to provide data on their infection level for the host and the species of this nematode parasite that we could find in several species of commercial interest in the Canary Archipelago. Fish specimens (n = 172) from the Canary coasts were examined for parasites. In total, 495 larvae were identified; PCR was carried out for the entire ITS rDNA and cox2 mtDNA region, obtaining sixteen sequences for the entire ITS rDNA region and fifteen for the cox2 mtDNA, this being the first contribution of nucleotide sequences of species of fish caught from the Canary Islands. An overall prevalence of 25% was obtained in the fish analyzed, and five species of were identified, these being (s.s.), , and and the hybrid x . The results obtained in this study have relevance for public health, since the pathology will depend on the species of so it is important to know the health status of fish in the waters of the Canary Islands to assure a safer consumption and take adequate measures, in addition to the provision of epidemiological data.

摘要

该研究旨在对加那利群岛海岸商业鱼类中的幼虫进行分子鉴定,并提供有关其感染水平的数据,包括宿主以及我们在加那利群岛几种具有商业价值的鱼类中发现的这种线虫寄生虫的种类。对来自加那利海岸的鱼类样本(n = 172)进行了寄生虫检查。总共鉴定出495只幼虫;对整个ITS rDNA和cox2 mtDNA区域进行了PCR,获得了16个ITS rDNA区域的序列和15个cox2 mtDNA的序列,这是首次对从加那利群岛捕获的鱼类物种的核苷酸序列做出贡献。在所分析的鱼类中总体患病率为25%,并鉴定出5种,分别是(狭义的)、、和以及杂交种x。本研究获得的结果与公共卫生相关,因为病理学将取决于的种类,所以了解加那利群岛水域鱼类的健康状况对于确保更安全的消费并采取适当措施很重要,此外还能提供流行病学数据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9617/9559264/7d507f1244dd/animals-12-02634-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9617/9559264/80750a84cb88/animals-12-02634-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9617/9559264/2a5b2958acfe/animals-12-02634-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9617/9559264/7d507f1244dd/animals-12-02634-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9617/9559264/80750a84cb88/animals-12-02634-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9617/9559264/2a5b2958acfe/animals-12-02634-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9617/9559264/7d507f1244dd/animals-12-02634-g003.jpg

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