State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan Aquaculture Breeding Engineering Research Center, Hainan Academician Team Innovation Center, Hainan University, Haikou 570228, China.
Int J Mol Sci. 2022 Sep 23;23(19):11186. doi: 10.3390/ijms231911186.
Fish skin color is often strongly affected by background color. We hypothesized that the regulatory mechanism of variations in skin color in is linked to the background color. In this study, we conducted transcriptome analysis of cultured under different background colors to compare gene expression levels and the important signaling pathways. The RNA-seq analysis yielded 26,675 known mRNAs, 3278 novel mRNAs, and 3179 differentially expressed genes (DEGs). The DEGs related to melanin synthesis were screened out. Some key melanin-related genes were identified, specifically , , , , , , and . These DEGs were mainly involved in melanogenesis, including tyrosine metabolism, the Wnt signaling pathway, and the cAMP signaling pathway. The expression levels of some key genes were upregulated when background color deepened, such as , , and . The α-MSH/cAMP-dependent, Wnt/β-catenin, and PI3K/Akt signaling pathways were activated, resulting in the accumulation of intracellular . promoted melanin production by binding to the // promoter region. In the present study, we explored the molecular mechanism underlying the darkened skin color pattern of , providing a theoretical basis for the molecular mechanism underlying pigmentation in .
鱼类的皮肤颜色常常受到背景颜色的强烈影响。我们假设 的皮肤颜色变化的调节机制与背景颜色有关。在这项研究中，我们对不同背景颜色下培养的 进行了转录组分析，以比较基因表达水平和重要的信号通路。RNA-seq 分析产生了 26675 个已知的 mRNAs、3278 个新的 mRNAs 和 3179 个差异表达基因（DEGs）。筛选出与黑色素合成相关的 DEGs。鉴定出一些关键的黑色素相关基因，特别是 、 、 、 、 、 。这些 DEGs 主要参与黑色素生成，包括酪氨酸代谢、Wnt 信号通路和 cAMP 信号通路。当背景颜色加深时，一些关键基因的表达水平上调，如 、 、 。α-MSH/cAMP 依赖性、Wnt/β-catenin 和 PI3K/Akt 信号通路被激活，导致细胞内 的积累。 通过与 // 启动子区域结合促进黑色素生成。在本研究中，我们探讨了 皮肤颜色变暗的分子机制，为 色素沉着的分子机制提供了理论基础。
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