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用于减弱玻璃表面纳米拓扑结构以增强间充质干细胞分化的DNA/磁性纳米颗粒复合材料

DNA/Magnetic Nanoparticles Composite to Attenuate Glass Surface Nanotopography for Enhanced Mesenchymal Stem Cell Differentiation.

作者信息

Ishmukhametov Ilnur, Batasheva Svetlana, Rozhina Elvira, Akhatova Farida, Mingaleeva Rimma, Rozhin Artem, Fakhrullin Rawil

机构信息

Institute of Fundamental Medicine and Biology, Kazan Federal University, Kreml uramı 18, 420008 Kazan, Republic of Tatarstan, Russian Federation.

出版信息

Polymers (Basel). 2022 Jan 17;14(2):344. doi: 10.3390/polym14020344.

DOI:10.3390/polym14020344
PMID:35054750
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8779295/
Abstract

Mesenchymal stem cells (MSCs) have extensive pluripotent potential to differentiate into various cell types, and thus they are an important tool for regenerative medicine and biomedical research. In this work, the differentiation of hTERT-transduced adipose-derived MSCs (hMSCs) into chondrocytes, adipocytes and osteoblasts on substrates with nanotopography generated by magnetic iron oxide nanoparticles (MNPs) and DNA was investigated. Citrate-stabilized MNPs were synthesized by the chemical co-precipitation method and sized around 10 nm according to microscopy studies. It was shown that MNPs@DNA coatings induced chondrogenesis and osteogenesis in hTERT-transduced MSCs. The cells had normal morphology and distribution of actin filaments. An increase in the concentration of magnetic nanoparticles resulted in a higher surface roughness and reduced the adhesion of cells to the substrate. A glass substrate modified with magnetic nanoparticles and DNA induced active chondrogenesis of hTERT-transduced MSC in a twice-diluted differentiation-inducing growth medium, suggesting the possible use of nanostructured MNPs@DNA coatings to obtain differentiated cells at a reduced level of growth factors.

摘要

间充质干细胞（MSCs）具有广泛的多能分化潜能，可分化为多种细胞类型，因此它们是再生医学和生物医学研究的重要工具。在这项工作中，研究了经hTERT转导的脂肪来源间充质干细胞（hMSCs）在由磁性氧化铁纳米颗粒（MNPs）和DNA产生的纳米拓扑结构底物上向软骨细胞、脂肪细胞和成骨细胞的分化。通过化学共沉淀法合成了柠檬酸盐稳定的MNPs，根据显微镜研究，其尺寸约为10纳米。结果表明，MNPs@DNA涂层可诱导经hTERT转导的MSCs发生软骨生成和成骨生成。细胞具有正常的形态和肌动蛋白丝分布。磁性纳米颗粒浓度的增加导致表面粗糙度更高，并降低了细胞与底物的粘附力。用磁性纳米颗粒和DNA修饰的玻璃底物在两倍稀释的诱导分化生长培养基中诱导经hTERT转导的MSC发生活跃的软骨生成，这表明纳米结构的MNPs@DNA涂层可能用于在降低生长因子水平的情况下获得分化细胞。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6205/8779295/91d7d1192a97/polymers-14-00344-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6205/8779295/7769dc1db8db/polymers-14-00344-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6205/8779295/98cedb78d6c1/polymers-14-00344-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6205/8779295/95af715ea2b8/polymers-14-00344-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6205/8779295/8f1ae4c2a4d5/polymers-14-00344-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6205/8779295/bca4b33d915d/polymers-14-00344-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6205/8779295/71f71da06b59/polymers-14-00344-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6205/8779295/91d7d1192a97/polymers-14-00344-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6205/8779295/7769dc1db8db/polymers-14-00344-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6205/8779295/98cedb78d6c1/polymers-14-00344-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6205/8779295/95af715ea2b8/polymers-14-00344-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6205/8779295/8f1ae4c2a4d5/polymers-14-00344-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6205/8779295/bca4b33d915d/polymers-14-00344-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6205/8779295/71f71da06b59/polymers-14-00344-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6205/8779295/91d7d1192a97/polymers-14-00344-g007.jpg

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本文引用的文献

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