Mechanical Memory Impairs Adipose-Derived Stem Cell (ASC) Adipogenic Capacity After Long-Term Expansion.

INTRODUCTION

Adipose derived stem cells (ASCs) hold great promise for clinical applications such as soft tissue regeneration and for tissue models and are notably easy to derive in large numbers. Specifically, ASCs provide an advantage for models of adipose tissue, where they can be employed as tissue specific cells and for patient specific models. However, ASC expansion may unintentionally reduce adipogenic capacity due to the stiffness of tissue culture plastic (TCPS).

METHODS

Here, we expanded freshly isolated ASCs on soft and stiff substrates for 4 passages before adipogenic differentiation. At the last passage we swapped the substrate from stiff to soft, or soft to stiff to determine if short term exposure to a different substrate altered adipogenic capacity.

RESULTS

Expansion on stiff substrates reduced adipogenic capacity by 50% which was not rescued by swapping to a soft substrate for the last passage. Stiff substrates had greater nuclear area and gene expression of nesprin-2, a protein that mediates the tension of the nuclear envelope by tethering it to the actin cytoskeleton. Upon swapping to a soft substrate, the nuclear area was reduced but nesprin-2 levels did not fully recover, which differentially regulated cell commitment transcriptional factors.

CONCLUSION

Therefore, expansion on stiff substrates must be carefully considered when the end-goal of the expansion is for adipose tissue or soft tissue applications.