Laboratory of Physical Chemistry of Processes, Faculty of Sciences and Techniques, Hassan First University, B.P. 539, Settat 26000, Morocco.
Biomedical Research Center, Romanian Academy, Iași Branch, 8th Carol I Avenue, 700506 Iași, Romania.
Molecules. 2022 Oct 1;27(19):6477. doi: 10.3390/molecules27196477.
() has been historically used for medicinal purposes due to its biological activity against specific disorders. To investigate the antioxidant and the antiproliferativepotential of essential oil in vitro and in vivo, the chemical composition of the essential oil was analyzed by GC-MS. The in vivo antioxidant capacity was assessed on liver and kidney homogenate samples from mice subjected to acetaminophen-induced oxidative stress and treated with essential oil (600 and 12,000 mg/kg BW) for 14 days. The in vitro scavenging activity was assayed using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) and the reducing power methods. The cytotoxic effects against the HeLa cancer cell line were analyzed. The GC-MS analysis showed the presence of compounds, of which were identified as major constituents. The essential oil protected mice's liver and kidneys from acetaminophen-induced oxidative stress by enhancing antioxidant enzymes (catalase, superoxide dismutase, and glutathione) and lowering malondialdehyde levels. In vitro, the essential oil demonstrated low scavenging activity against DPPH (IC = 2.00 ± 0.05 mg/mL) and modest reducing power (EC = 0.963 ± 0.006 mg/mL). The growth of the HeLa cells was also reduced by the essential oil with an inhibition rate of 83.58% at 95 µg/mL. Current results reveal significant antioxidant and antiproliferative effects in a dose-dependent manner and suggest that essential oil could be useful in formulations for cancer therapy.
() 由于其对特定疾病的生物活性，在历史上一直被用于药用目的。为了研究精油的抗氧化和抗增殖潜力，通过 GC-MS 分析了精油的化学成分。通过对乙酰氨基酚诱导的氧化应激小鼠的肝和肾匀浆样本进行体内抗氧化能力评估，并分别用 精油（600 和 12,000 mg/kg BW）处理 14 天。使用 1,1-二苯基-2-苦基肼（DPPH）和还原力法测定体外清除活性。分析了对 HeLa 癌细胞系的细胞毒性作用。GC-MS 分析表明存在 种化合物，其中 种被鉴定为主要成分。精油通过增强抗氧化酶（过氧化氢酶、超氧化物歧化酶和谷胱甘肽）和降低丙二醛水平来保护小鼠的肝脏和肾脏免受乙酰氨基酚诱导的氧化应激。在体外，精油对 DPPH（IC = 2.00 ± 0.05 mg/mL）的清除活性较低，还原力较弱（EC = 0.963 ± 0.006 mg/mL）。HeLa 细胞的生长也被精油抑制，在 95 µg/mL 时抑制率为 83.58%。目前的结果表明，精油具有显著的抗氧化和增殖抑制作用，呈剂量依赖性，并表明精油可用于癌症治疗的制剂。
