Department of Forensic Medicine, Wroclaw Medical University, 4 J. Mikulicza-Radeckiego Street, 50345 Wroclaw, Poland.
Institute of Toxicology Research, 45 Kasztanowa Street, 55093 Borowa, Poland.
Molecules. 2022 Oct 3;27(19):6534. doi: 10.3390/molecules27196534.
The aim of this study was establishment of an UHPLC-QqQ-MS/MS method for the deter-mination of misoprostol acid in biological specimens in cases of pharmacological abortions. Forensic toxicological examination was performed in three different biological samples (whole blood, placenta and fetal liver). The validation parameters of the method were as follows: limit of detection: 25 pg/mL; limit of quantification: 50 pg/mL, coefficient of determination: >0.999 (R2), intra- and interday accuracy and precision: not greater than 13.7%. The recovery and matrix effect were in the range of 88.3−95.1% and from −11.7 to −4.9%, respectively. Toxicological analysis of the mother’s blood (collected two days after pregnancy termination) did not reveal any abortifacients; however, misoprostol acid was found in the placenta (793 pg/g) and fetal liver (309 pg/g). The second case involved a fetus found near a garbage container. The concentration of misoprostol acid in the placenta was 2332 pg/g. In the presented study, an extensive literature review of misoprostol pharmacokinetics studies was performed. To our knowledge, the UHPLC-QqQ-MS/MS technique presented in this paper is the first quantitative method applied for forensic toxicological purposes. In addition, postmortem concentrations of misoprostol acid in miscarried fetuses due to illegal abortions were reported for the first time.
本研究的目的是建立一种 UHPLC-QqQ-MS/MS 方法，用于检测药理学流产中生物标本中的米索前列醇酸。进行了法医毒理学检查在三种不同的生物样本（全血、胎盘和胎肝）中。该方法的验证参数如下：检测限：25 pg/mL；定量限：50 pg/mL，决定系数：>0.999（R2），日内和日间精密度和准确度：不超过 13.7%。回收率和基质效应分别在 88.3-95.1%和-11.7 至-4.9%的范围内。对母亲终止妊娠两天后采集的血液进行毒理学分析未发现任何堕胎药；然而，在胎盘（793 pg/g）和胎肝（309 pg/g）中发现了米索前列醇酸。第二个案例涉及在垃圾桶附近发现的胎儿。胎盘中的米索前列醇酸浓度为 2332 pg/g。在本研究中，对米索前列醇药代动力学研究进行了广泛的文献回顾。据我们所知，本文提出的 UHPLC-QqQ-MS/MS 技术是首次应用于法医毒理学目的的定量方法。此外，还首次报道了因非法堕胎而流产的胎儿死后米索前列醇酸的浓度。
