Institute for Control of Chinese Traditional Medicine and Ethnic Medicine, National Institutes for Food and Drug Control, Beijing 102600, China.
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China.
Molecules. 2022 Oct 10;27(19):6760. doi: 10.3390/molecules27196760.
In recent years, the hepatotoxicity of Polygoni Multiflora Radix (PMR) has attracted increased research interest. Some studies suggest that anthraquinone may be the main hepatotoxic component. Most of the relevant studies have focused on the mononuclear anthraquinone component rather than binuclear anthraquinones. The hepatotoxicity of dinuclear anthraquinone (dianthrone) was investigated in a cell-based model. Next, a method for the determination of six free and total dianthonones in PMR and PMR Praeparata (PMRP) was established using ultra-high-performance liquid chromatography triple quadrupole mass spectrometry (UPLC-QQQ-MS/MS), which was then used to analyze the collected samples. The data show that four binuclear anthraquinone compounds were hepatotoxic and may be potential toxicity indicators for the safety evaluation of PMR and PMRP. Herein, we provide a theoretical basis for the improvement of PMRP quality standards.
近年来，何首乌的肝毒性引起了越来越多的研究关注。一些研究表明，蒽醌可能是主要的肝毒性成分。大多数相关研究都集中在单核蒽醌成分上，而不是双蒽醌上。本研究采用基于细胞的模型研究了双蒽醌（二蒽酮）的肝毒性。接下来，采用超高效液相色谱三重四极杆质谱（UPLC-QQQ-MS/MS）建立了测定何首乌和制何首乌中 6 种游离和总二蒽酮的方法，并对采集的样品进行了分析。研究数据表明，4 种双蒽醌化合物具有肝毒性，可能是何首乌和制何首乌安全性评价的潜在毒性指标。本研究为提高制何首乌质量标准提供了理论依据。
