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基于超高效液相色谱/四极杆-轨道阱质谱代谢组学及相关性分析快速发现[具体事物]中的潜在毒性化合物

Rapid Discovery of the Potential Toxic Compounds in by UHPLC/Q-Orbitrap-MS-Based Metabolomics and Correlation Analysis.

作者信息

Han Lifeng, Wang Piao, Wang Yulan, Zhao Qianyu, Zheng Fang, Dou Zhiying, Yang Wenzhi, Hu Limin, Liu Caixiang

机构信息

Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin Key Laboratory of TCM Chemistry and Analysis, Tianjin University of Traditional Chinese Medicine, Tianjin, China.

CAS Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Centre for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, the Chinese Academy of Sciences, Wuhan, China.

出版信息

Front Pharmacol. 2019 Apr 16;10:329. doi: 10.3389/fphar.2019.00329. eCollection 2019.

DOI:10.3389/fphar.2019.00329
PMID:31057397
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6477936/
Abstract

The dry roots of (PM), involving both the raw and processed materials, are widely used as the traditional Chinese medicine for treating various diseases in China. Hepatotoxicity has been occasionally reported in patients who consume PM. Unfortunately, no definite criteria are currently available regarding the processing technology of PM for reduction the toxicity. In this work, we aimed to investigate the variations of PM metabolite profiles induced by different processing technologies by UHPLC/Q-Orbitrap-MS and multivariate statistical analysis, and to discover the potential toxic compounds by correlating the cytotoxicity of L02 cell with the contents of metabolites in raw and processed PM samples. We could identify two potential toxic compounds, emodin-8--glucoside and torachrysone--hexose, which could be selected as the toxic markers to evaluate different processing methods. The results indicated all processed PM samples could decrease the cytotoxicity on L02 cell. The best processing technology for PM process was to steam PM in black soybean decoction (BD-PM) for 24 h.

摘要

何首乌（PM）的干燥根，包括生品和炮制品，在中国被广泛用作治疗各种疾病的传统中药。食用何首乌的患者偶尔会报告肝毒性。不幸的是，目前尚无关于降低何首乌毒性的炮制技术的确切标准。在这项工作中，我们旨在通过超高效液相色谱/四极杆-轨道阱质谱联用（UHPLC/Q-Orbitrap-MS）和多元统计分析研究不同炮制技术引起的何首乌代谢物谱变化，并通过将L02细胞的细胞毒性与生何首乌和炮制何首乌样品中的代谢物含量相关联来发现潜在的有毒化合物。我们可以鉴定出两种潜在的有毒化合物，大黄素-8-O-葡萄糖苷和大黄素甲醚-己糖，它们可以作为毒性标志物来评估不同的炮制方法。结果表明，所有炮制的何首乌样品均可降低对L02细胞的细胞毒性。何首乌炮制的最佳工艺是在黑豆汤中蒸何首乌（BD-PM）24小时。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfe7/6477936/976435987076/fphar-10-00329-g008.jpg
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