School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.
Molecules. 2021 Jun 29;26(13):3977. doi: 10.3390/molecules26133977.
Polygoni Multiflori Radix Praeparata (PMRP), as the processed product of tuberous roots of Thunb., is one of the most famous traditional Chinese medicines, with a long history. However, in recent years, liver adverse reactions linked to PMRP have been frequently reported. Our work attempted to investigate the chemical constituents of PMRP for clinical research and safe medication. In this study, an effective and rapid method was established to separate and characterize the constituents in PMRP by combining ultra-high performance liquid chromatography with hybrid quadrupole-orbitrap mass spectrometry (UHPLC-Q-Exactive Orbitrap-MS). Based on the accurate mass measurements for molecular and characteristic fragment ions, a total of 103 compounds, including 24 anthraquinones, 21 stilbenes, 15 phenolic acids, 14 flavones, and 29 other compounds were identified or tentatively characterized. Forty-eight compounds were tentatively characterized from PMRP for the first time, and their fragmentation behaviors were summarized. There were 101 components in PMRP ethanol extract (PMRPE) and 91 components in PMRP water extract (PMRPW). Simultaneously, the peak areas of several potential xenobiotic components were compared in the detection, which showed that PMRPE has a higher content of anthraquinones and stilbenes. The obtained results can be used in pharmacological and toxicological research and provided useful information for further in vitro and in vivo studies.
制何首乌（PMRP）是蓼科植物何首乌的块根经过炮制后的产物，是一种具有悠久历史的著名中药。然而，近年来，与 PMRP 相关的肝不良反应频繁报道。我们的工作旨在研究 PMRP 的化学成分，为临床研究和安全用药提供依据。本研究采用超高效液相色谱-四级杆轨道阱质谱联用技术（UHPLC-Q-Exactive Orbitrap-MS），建立了一种快速有效的方法，用于分离和鉴定 PMRP 中的成分。基于精确质量测量的分子离子和特征碎片离子，共鉴定或推测出 103 种化合物，包括 24 种蒽醌类、21 种二苯乙烯类、15 种酚酸类、14 种黄酮类和 29 种其他化合物。首次从 PMRP 中鉴定出 48 种化合物，并总结了它们的裂解行为。PMRP 乙醇提取物（PMRPE）中有 101 种成分，PMRP 水提取物（PMRPW）中有 91 种成分。同时，比较了检测中几种潜在外源成分的峰面积，结果表明 PMRPE 中蒽醌类和二苯乙烯类成分含量较高。所得结果可用于药理学和毒理学研究，并为进一步的体外和体内研究提供了有用信息。
