Iranifam Mortaza, Feizzadeh Maryam
Department of Chemistry, Faculty of Science, University of Maragheh, Maragheh, Iran.
Luminescence. 2022 Dec;37(12):2083-2089. doi: 10.1002/bio.4395. Epub 2022 Oct 28.
In this research, β-MnOOH nanoplates (NPLs) were hydrothermally produced and then identified using several spectroscopic methods. The β-MnOOH NPLs were used to catalyze the chemiluminescence (CL) reaction of NaHCO -H O . To validate the capability of the CL reaction for pharmaceutical analysis, the CL reaction of β-MnOOH NPLs-NaHCO -H O reaction was exploited to develop a new method of measuring antibiotics named amoxicillin (AMX) and salbutamol sulfate (SLB). This method is based on the attenuating β-MnOOH NPLs-NaHCO -H O CL reaction by the antibiotics. Calibration curves were linear in the range 3.00 × 10 to 1.00 × 10 mol L for AMX and in the range 1.00 × 10 to 1.00 × 10 mol L for SLB. The limits of detections obtained using the CL method for AMX and SLB were 8.90 × 10 mol L and 5.60 × 10 mol L , respectively. The relative standard deviations for AMX and SLB, at the 5.00 × 10 mol L concentration, were 2.44% and 2.57% (n = 5), respectively. The study of the effect of foreign species showed that the CL method developed has the appropriate selectivity for AMX and SLB. The success of the CL method in actual samples analysis was demonstrated by accurately measuring the selected antibiotics in the pharmaceutical formulations.
在本研究中，通过水热法制备了β-氢氧化锰纳米片（NPLs），然后使用多种光谱方法对其进行了鉴定。β-氢氧化锰纳米片用于催化NaHCO₃-H₂O₂的化学发光（CL）反应。为验证该CL反应在药物分析中的能力，利用β-氢氧化锰纳米片-NaHCO₃-H₂O₂反应的CL反应开发了一种测定抗生素阿莫西林（AMX）和硫酸沙丁胺醇（SLB）的新方法。该方法基于抗生素对β-氢氧化锰纳米片-NaHCO₃-H₂O₂ CL反应的衰减。AMX的校准曲线在3.00×10⁻⁸至1.00×10⁻⁶mol·L⁻¹范围内呈线性，SLB的校准曲线在1.00×10⁻⁷至1.00×10⁻⁵mol·L⁻¹范围内呈线性。使用CL法测定AMX和SLB的检测限分别为8.90×10⁻⁹mol·L⁻¹和5.60×10⁻⁹mol·L⁻¹。在5.00×10⁻⁷mol·L⁻¹浓度下，AMX和SLB的相对标准偏差分别为2.44%和2.57%（n = 5）。对外来物种影响的研究表明，所开发的CL法对AMX和SLB具有适当的选择性。通过准确测定药物制剂中选定的抗生素，证明了CL法在实际样品分析中的成功。
