Department of Chemistry, Faculty of Basic Sciences, University of Maragheh, Maragheh, Iran.
Department of Analytical Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran.
Luminescence. 2024 Apr;39(4):e4745. doi: 10.1002/bio.4745.
This study introduces a novel chemiluminescence (CL) approach utilizing FeS nanosheets (NSs) catalyzed luminol-O CL reaction for the measurement of three pharmaceuticals, namely venlafaxine hydrochloride (VFX), imipramine hydrochloride (IPM), and cefazolin sodium (CEF). The CL method involved the phenomenon of quenching induced by the pharmaceuticals in the CL reaction. To achieve the most quenching efficacy of the pharmaceuticals in the CL reaction, the concentrations of reactants comprising luminol, NaOH, and FeS NSs were optimized accordingly. The calibration curves demonstrated exceptional linearity within the concentration range spanning from 4.00 × 10 to 1.00 × 10 mol L, 1.00 × 10 to 1.00 × 10 mol L, and 4.00 × 10 to 2.00 × 10 mol L with detection limits (3σ) of 3.54 × 10, 1.08 × 10, and 2.63 × 10 mol L for VFX, IPM, and CEF, respectively. This study synthesized FeS NSs using a facile hydrothermal approach, and then the synthesized FeS NSs were subjected to a comprehensive characterization using a range of spectroscopic methods. The proposed CL method was effective in measuring the aforementioned pharmaceuticals in pharmaceutical formulations as well as different water samples. The mechanism of the CL system has been elucidated.
本研究提出了一种利用 FeS 纳米片（NSs）催化鲁米诺-O CL 反应的新型化学发光（CL）方法，用于测量三种药物，即盐酸文拉法辛（VFX）、盐酸丙咪嗪（IPM）和头孢唑林钠（CEF）。CL 方法涉及药物在 CL 反应中引起的猝灭现象。为了在 CL 反应中达到药物的最大猝灭效果，相应地优化了包括鲁米诺、NaOH 和 FeS NSs 在内的反应物的浓度。校准曲线在 4.00 × 10 至 1.00 × 10 mol L、1.00 × 10 至 1.00 × 10 mol L 和 4.00 × 10 至 2.00 × 10 mol L 的浓度范围内表现出优异的线性关系，检测限（3σ）分别为 3.54 × 10、1.08 × 10 和 2.63 × 10 mol L，用于 VFX、IPM 和 CEF。本研究使用简便的水热法合成了 FeS NSs，然后使用一系列光谱方法对合成的 FeS NSs 进行了全面表征。所提出的 CL 方法可有效测量药物制剂和不同水样中的上述药物。还阐明了 CL 体系的机制。
