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炎症过程中鼠单核细胞趋化蛋白-1 表达的生物发光成像。

Bioluminescence imaging of mouse monocyte chemoattractant protein-1 expression in inflammatory processes.

机构信息

School of Life Sciences and Technology, Tongji University, Shanghai 200092, China.

Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.

出版信息

Acta Biochim Biophys Sin (Shanghai). 2022 Oct 25;54(10):1507-1517. doi: 10.3724/abbs.2022143.

DOI:10.3724/abbs.2022143
PMID:36239355
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9828394/
Abstract

Monocyte chemoattractant protein-1 (MCP-1) plays a crucial role in various inflammatory diseases. To reveal the impact of MCP-1 during diseases and to develop anti-inflammatory agents, we establish a transgenic mouse line. The firefly luciferase gene is incorporated into the mouse genome and driven by the endogenous promoter. A bioluminescence photographing system is applied to monitor luciferase levels in live mice during inflammation, including lipopolysaccharide-induced sepsis, concanavalin A-induced T cell-dependent liver injury, CCl -induced acute hepatitis, and liver fibrosis. The results demonstrate that the luciferase signal induced in inflammatory processes is correlated with endogenous MCP-1 expression in mice. Furthermore, the expressions of and the luciferase gene are dramatically inhibited by administration of the anti-inflammatory drug dexamethasone in a septicemia model. Our results suggest that the transgenic MCP-1-Luc mouse is a useful model to study MCP-1 expression in inflammation and disease and to evaluate the efficiency of anti-inflammatory drugs .

摘要

单核细胞趋化蛋白-1（MCP-1）在各种炎症性疾病中起着至关重要的作用。为了揭示 MCP-1 在疾病中的作用并开发抗炎药物，我们建立了一个转基因小鼠品系。萤火虫荧光素酶基因被整合到小鼠基因组中，并由内源性启动子驱动。应用生物发光成像系统监测活体小鼠在炎症过程中的荧光素酶水平，包括脂多糖诱导的败血症、刀豆蛋白 A 诱导的 T 细胞依赖性肝损伤、CCl4 诱导的急性肝炎和肝纤维化。结果表明，在炎症过程中诱导的荧光素酶信号与小鼠内源性 MCP-1 的表达相关。此外，在败血症模型中，抗炎药物地塞米松的给药显著抑制 和荧光素酶基因的表达。我们的结果表明，转基因 MCP-1-Luc 小鼠是研究炎症和疾病中 MCP-1 表达以及评估抗炎药物疗效的有用模型。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e616/9828394/f46e4fa5fb44/ABBS-2022-058-t9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e616/9828394/41196eba5f60/ABBS-2022-058-t1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e616/9828394/e5a0a6f21826/ABBS-2022-058-t2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e616/9828394/5362860fe055/ABBS-2022-058-t3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e616/9828394/bb7a93923649/ABBS-2022-058-t4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e616/9828394/9a298841bdf9/ABBS-2022-058-t5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e616/9828394/32f2f76a33b0/ABBS-2022-058-t6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e616/9828394/6b54bb0cf01f/ABBS-2022-058-t7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e616/9828394/740230bd3821/ABBS-2022-058-t8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e616/9828394/f46e4fa5fb44/ABBS-2022-058-t9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e616/9828394/41196eba5f60/ABBS-2022-058-t1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e616/9828394/e5a0a6f21826/ABBS-2022-058-t2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e616/9828394/5362860fe055/ABBS-2022-058-t3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e616/9828394/bb7a93923649/ABBS-2022-058-t4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e616/9828394/9a298841bdf9/ABBS-2022-058-t5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e616/9828394/32f2f76a33b0/ABBS-2022-058-t6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e616/9828394/6b54bb0cf01f/ABBS-2022-058-t7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e616/9828394/740230bd3821/ABBS-2022-058-t8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e616/9828394/f46e4fa5fb44/ABBS-2022-058-t9.jpg

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