Division of Orthodontics, School of Dental Medicine, UConn Health, Farmington, CT, USA.
Department of Developmental Sciences, Marquette University School of Dentistry, Milwaukee, WI, USA.
Cartilage. 2022 Dec;13(4):171-183. doi: 10.1177/19476035221109229. Epub 2022 Oct 14.
To characterize the effects of parathyroid hormone (PTH) and alendronate (Alend) on the osteochondral tissue of temporomandibular joint (TMJ).
Ninety-six male and female transgenic reporter mice, 4 to 5 weeks old were divided into 6 groups: (1) Control group: Saline was injected daily for 14 days; (2) PTH: PTH was injected daily for 14 days; (3) Alend: Alend was injected every alternate days for 14 days; (4) Combined PTH and Alend: PTH was injected daily and Alend injected every alternate days for 14 days; (5) PTH then Alend: PTH was injected daily for 14 days followed by Alend injections in alternate days for 14 days; and (6) PTH wait Alend: PTH was injected daily for 14 days. There was a waiting period of 1 week before administration of Alend in alternate days for 14 days. Mice were injected with 5-ethnyl-2'-deoxyuridine (EdU), 48 and 24 hours prior to euthanization.
There was significant increase in bone volume and decrease in osteoclastic activity in groups in which Alend was administered after PTH in both gender. There was significant increase in cartilage thickness with PTH or Alend alone in females, whereas in males, PTH alone led to increase in cartilage thickness. Chondrocyte apoptosis was significantly decreased with PTH or Alend alone in both male and female. Matrix metallopeptidase 13, and aggreganase-2 (ADAMTS5) expression were significantly decreased with PTH and Alend alone in both gender.
PTH and Alend administration causes anabolic effects in the osteochondral tissue of TMJ.
研究甲状旁腺激素（PTH）和阿仑膦酸钠（Alend）对颞下颌关节（TMJ）的骨软骨组织的影响。
将 96 只 4 至 5 周龄的雄性和雌性转基因报告小鼠分为 6 组：（1）对照组：每天注射生理盐水 14 天；（2）PTH 组：每天注射 PTH 14 天；（3）Alend 组：每隔一天注射 Alend 14 天；（4）PTH+Alend 联合组：每天注射 PTH 并每隔一天注射 Alend 14 天；（5）PTH 后 Alend 组：每天注射 PTH 14 天后，每隔一天注射 Alend 14 天；（6）PTH 等待 Alend 组：每天注射 PTH 14 天后，等待 1 周再开始每隔一天注射 Alend 14 天。在处死前，小鼠分别于 48 小时和 24 小时前注射 5-乙氧嘧啶-2'-脱氧尿苷（EdU）。
在两性中，PTH 后给予 Alend 的组中，骨体积增加，破骨细胞活性降低。PTH 或 Alend 单独使用时，雌性的软骨厚度显著增加，而雄性的软骨厚度仅在 PTH 单独使用时增加。PTH 或 Alend 单独使用时，两性的软骨细胞凋亡均显著减少。基质金属蛋白酶 13 和聚集酶-2（ADAMTS5）的表达在两性中均随 PTH 和 Alend 的单独使用而显著降低。
PTH 和 Alend 的给药会导致 TMJ 骨软骨组织的合成代谢作用。
