College of Materials Science and Engineering, College of Chemistry and Chemical Engineering, Shandong Sino-Japanese-Japanese Center for Collaborative Research of Carbon Nanomaterials, Instrumental Analysis Center of Qingdao University, Institute of Biomedical Engineering, Qingdao University, Qingdao, 266071, Shandong, China.
Anal Bioanal Chem. 2022 Sep;414(23):6753-6760. doi: 10.1007/s00216-022-04235-9. Epub 2022 Aug 1.
Nanomaterial-derived quantum dots (QDs) are excellent electrochemiluminescence (ECL) luminophores and play an important role in optical sensing due to their excellent water solubility, good biocompatibility and tunable molecular size. In this work, a novel strategy was designed to form nano-hybrid TiC QDs-AuNPs in situ as a luminophore based on the unique reducibility of TiC QDs, which showed remarkable and stable ECL performance. Here, AuNPs were formed in situ without the addition of reducing agents and stabilizers, leading to threefold enhancement of the ECL signal of TiC QDs due to their excellent charge transfer capability. Meanwhile, TiC QDs-AuNPs with abundant Ti atoms also acted as recognition units. Through skillful combination with hybridization chain reaction (HCR) to expose more phosphate, an ECL platform was constructed to detect polynucleotide kinase (PNK) with good specificity and sensitivity. A lower limit of detection limit of 2.7×10 U mL was achieved, with a wide linear relationship ranging from 0.0001 to 10 U mL. This novel strategy provides a guide for the application of nano-hybrid TiC QDs-AuNPs as a luminophore in the field of ECL bioanalysis. Novel in situ-formed nano-hybrid TiC QDs-AuNPs were prepared as a luminophore, with threefold enhancement of the ECL signal of TiC QDs.
纳米材料衍生的量子点 (QDs) 是出色的电致化学发光 (ECL) 发光体，由于其出色的水溶性、良好的生物相容性和可调的分子尺寸，在光学传感中发挥着重要作用。在这项工作中，设计了一种新策略，通过利用 TiC QDs 的独特还原性能，原位形成纳米杂化 TiC QDs-AuNPs 作为发光体，从而表现出显著且稳定的 ECL 性能。在这里，无需添加还原剂和稳定剂即可原位形成 AuNPs，由于其出色的电荷转移能力，使 TiC QDs 的 ECL 信号增强了三倍。同时，富含 Ti 原子的 TiC QDs-AuNPs 也充当了识别单元。通过巧妙地与杂交链式反应 (HCR) 结合，暴露更多的磷酸盐，构建了一个 ECL 平台，用于检测多核苷酸激酶 (PNK)，具有良好的特异性和灵敏度。检测限达到 2.7×10 U mL，线性关系范围从 0.0001 到 10 U mL 很宽。该新策略为纳米杂化 TiC QDs-AuNPs 作为 ECL 生物分析领域的发光体的应用提供了指导。制备了新型原位形成的纳米杂化 TiC QDs-AuNPs 作为发光体，使 TiC QDs 的 ECL 信号增强了三倍。
