突尼斯和乙醇提取物的生物活性及作用方式的测定。

Biological activities and determination of the mode of action of Tunisian and ethanolic extracts.

机构信息

National Research Institute of Rural Engineering, Water and Forests (INRGREF), LR11INRGREF0 Laboratory of Management and Valorization of Forest Resources, Carthage University, Ariana, Tunisia.

Laboratory of Epidemiology and Veterinary Microbiology, Group of Bacteriology and Biotechnology Development, Institut Pasteur de Tunis, Belvédère, Tunis.

出版信息

Int J Environ Health Res. 2024 Jan;34(1):127-137. doi: 10.1080/09603123.2022.2133096. Epub 2022 Oct 15.

DOI:10.1080/09603123.2022.2133096

PMID:36242557

Abstract

This study was designed to evaluate the antioxidant and antimicrobial activity of ethanolic extracts (EEs) of and . showed the highest values of phenolic compounds. Further, it was shown that EE of displayed the highest DPPH (IC50 = 8.3 ± 1.08 mg/mL). The chemical profiles demonstrated a total of 12 and 13 phenolic compounds for and , respectively. EEs of both plants possessed broad-spectrum antimicrobial activity when tested against and . Treatment of studied strains with these extracts at their MICs reduced considerably the bacterial viability. The extracts did not induce total bacterial lysis, as determined by the measurement of optical density at 620 nm. Strains treated with EEs at Minimum Inhibitory Concentrations showed significant loss of tolerance to NaCl. Our results contribute to understanding the antibacterial mechanism of ethanolic extracts of the studied medicinal plants.

摘要

本研究旨在评估和乙醇提取物（EEs）的抗氧化和抗菌活性。显示出最高的酚类化合物含量。此外，研究表明的 EE 表现出最高的 DPPH（IC50 = 8.3 ± 1.08 mg/mL）。化学分析显示，和中分别含有 12 种和 13 种酚类化合物。当测试对和时，两种植物的 EE 均具有广谱抗菌活性。在 MIC 下用这些提取物处理研究菌株，可显著降低细菌活力。通过测量 620nm 处的光密度，提取物未诱导细菌完全裂解。在最低抑菌浓度下用 EE 处理的菌株对 NaCl 的耐受性显著降低。我们的结果有助于理解研究药用植物乙醇提取物的抗菌机制。