Shen Yesheng, Yang Yudong, Zhao Jun, Geng Ningwei, Liu Kuihao, Zhao Yiran, Wang Fangkun, Liu Sidang, Li Ning, Meng Fanliang, Liu Mengda
School of Animal Science and Technology, Shandong Agricultural University, Tai'an, China.
Huayun (Shandong) Inspection and Quarantine Service Co., Tai'an, China.
Front Vet Sci. 2022 Oct 3;9:1015717. doi: 10.3389/fvets.2022.1015717. eCollection 2022.
Responsible for the acute infectious disease porcine epidemic diarrhea (PED), PED virus (PEDV) induces severe diarrhea and high mortality in infected piglets and thus severely harms the productivity and economic efficiency of pig farms. In our study, we aimed to investigate and analyze the recent status and incidence pattern of PEDV infection in some areas of Shandong Province, China. We collected 176 clinical samples of PED from pig farms in different regions of Shandong Province during 2019-2021. PEDV, TGEV, and PORV were detected using RT-PCR. The full-length sequences of positive PEDV S genes were amplified, the sequences were analyzed with MEGA X and DNAStar, and a histopathological examination of typical PEDV-positive cases was performed. RT-PCR revealed positivity rates of 37.5% (66/176) for PEDV, 6.82% (12/176) for transmissible gastroenteritis virus, and 3.98% (7/176) for pig rotavirus. The test results for the years 2019, 2020, and 2021 were counted separately, PEDV positivity rates for the years were 34.88% (15/43), 39.33% (35/89), and 36.36% (16/44), respectively. Histopathological examination revealed atrophied, broken, and detached duodenal and jejunal intestinal villi, as typical of PED, and severe congestion of the intestinal submucosa. Moreover, the results of our study clearly indicate that the G2 subtype is prevalent as the dominant strain of PEDV in Shandong Province, where its rates of morbidity and mortality continue to be high. Based on a systematic investigation and analysis of PEDV's molecular epidemiology across Shandong Province, our results enrich current epidemiological data regarding PEDV and provide some scientific basis for preventing and controlling the disease.
猪流行性腹泻病毒（PEDV）是引起急性传染病猪流行性腹泻（PED）的病原体，可导致感染仔猪严重腹泻和高死亡率，从而严重损害猪场的生产力和经济效益。在本研究中，我们旨在调查和分析中国山东省部分地区PEDV感染的现状和发病模式。我们在2019 - 2021年期间从山东省不同地区的猪场收集了176份PED临床样本。使用RT-PCR检测PEDV、传染性胃肠炎病毒（TGEV）和猪轮状病毒（PORV）。扩增阳性PEDV S基因的全长序列，用MEGA X和DNAStar分析序列，并对典型的PEDV阳性病例进行组织病理学检查。RT-PCR结果显示，PEDV阳性率为37.5%（66/176），传染性胃肠炎病毒阳性率为6.82%（12/176），猪轮状病毒阳性率为3.98%（7/176）。分别统计2019年、2020年和2021年的检测结果，这几年的PEDV阳性率分别为34.88%（15/43）、39.33%（35/89）和36.36%（16/44）。组织病理学检查显示十二指肠和空肠肠绒毛萎缩、断裂和脱落，这是PED的典型表现，肠黏膜下层严重充血。此外，我们的研究结果清楚地表明，G2亚型作为山东省PEDV的优势毒株流行，其发病率和死亡率仍然很高。基于对山东省PEDV分子流行病学的系统调查和分析，我们的结果丰富了当前关于PEDV的流行病学数据，并为该病的预防和控制提供了一些科学依据。
