Liu Lu, Xiao Zunsheng, Ding Wencui, Wen Chao, Ge Chao, Xu Ke, Cao Sumin
Department of Stomatology, Cangzhou Central Hospital Cangzhou, Hebei, China.
The First Department of Endocrine Diabetes, Cangzhou Central Hospital Cangzhou, Hebei, China.
Am J Transl Res. 2022 Sep 15;14(9):6627-6637. eCollection 2022.
To investigate the expression of single-stranded microRNAs (miRNAs) in serum and gingival crevicular fluid of patients with type 2 diabetes mellitus (T2DM) complicated by periodontal disease and its correlation with inflammatory factors.
Twenty-six periodontitis patients without diabetes mellitus (periodontal group), 24 patients with T2DM (T2DM group), 22 patients with both T2DM and periodontal disease (comorbid group), and 25 healthy individuals without a history of periodontal disease (healthy group) were recruited respectively. Serum and gingival crevicular fluid specimens were collected to detect the expression levels of miRNAs and inflammatory factors including serum tumor necrosis factor-α (TNF-α), interleukin-10 (IL-10), and transforming growth factor-β (TGF-β), and their correlations were also investigated.
Eleven miRNAs were detected in the gingival crevicular tissue of all subjects. The expression of miR-223 and miR-200b in serum and gingival crevicular fluid was elevated higher in the comorbid group than in the other three groups (.05), and their expressions in gingival crevicular fluid contributed to the differential diagnosis of periodontal disease from diabetes mellitus comorbid with periodontal disease (<0.05). Gingival crevicular fluid miR-223 expression was positively associated with TNF-α, clinical attachment loss (CAL), and probing pocket depth (PPD) in the periodontal group, while negatively associated with IL-10 (<.05), and so was gingival crevicular fluid miR-200b expression with TNF-α, CAL, and PPD (<0.). In the comorbid group, gingival crevicular fluid miR-223 expression showed a positive correlation with TNF-α, CAL, and PPD (<0.05), and so did gingival crevicular fluid miR-200b expression with TNF-α, CAL, and PPD (<0.05).
Our findings indicate a close link between the levels of miR-223 and miR-200b in serum and gingival crevicular fluid and susceptibility to T2DM as well as the pathogenesis of periodontal disease.
探讨2型糖尿病（T2DM）合并牙周病患者血清和龈沟液中单链微小RNA（miRNA）的表达及其与炎症因子的相关性。
分别招募26例无糖尿病的牙周炎患者（牙周组）、24例T2DM患者（T2DM组）、22例T2DM合并牙周病患者（合并组）和25例无牙周病病史的健康个体（健康组）。采集血清和龈沟液标本，检测miRNA和炎症因子的表达水平，包括血清肿瘤坏死因子-α（TNF-α）、白细胞介素-10（IL-10）和转化生长因子-β（TGF-β），并研究它们之间的相关性。
在所有受试者的龈沟组织中检测到11种miRNA。合并组血清和龈沟液中miR-223和miR-200b的表达高于其他三组（P<0.05），它们在龈沟液中的表达有助于牙周病与合并牙周病的糖尿病的鉴别诊断（P<0.05）。牙周组龈沟液miR-223表达与TNF-α、临床附着丧失（CAL）和探诊深度（PPD）呈正相关，与IL-10呈负相关（P<0.05），龈沟液miR-200b表达与TNF-α、CAL和PPD也呈正相关（P<0.05）。在合并组中，龈沟液miR-223表达与TNF-α、CAL和PPD呈正相关（P<0.05），龈沟液miR-200b表达与TNF-α、CAL和PPD也呈正相关（P<0.05）。
我们的研究结果表明，血清和龈沟液中miR-223和miR-200b的水平与T2DM易感性以及牙周病发病机制之间存在密切联系。
