Department of Parasitology and Mycology, Qazvin University of Medical Sciences, Qazvin, Iran.
Student Research Committee, Qazvin University of Medical Sciences, Qazvin, Iran.
Infect Disord Drug Targets. 2020;20(5):737-742. doi: 10.2174/1871526519666191009143043.
Fascioliasis and dicrocoeliasis are the most frequent zoonotic diseases with increasing human health problems in different parts of Iran. Two species, Fasciola hepatica (F. hepatica) and Fasciola gigantica (F. gigantica), are spread in the country. Molecular approaches have a decisive role in identifying both the species. The aim of this study was to detect Fasciola spp. and Dicrocoelium spp. by amplifying the ITS-2 and 28S rDNA gene sequence.
Overall, 30 infected liver samples were collected from the livestock of Qazvin, Iran. The adult flukes were collected from different livestock. DNA extraction and PCR amplification of ribosomal RNA gene region (ITS2) and 28S rDNA gene fragment were conducted and a phylogenetic tree was constructed.
All the isolates obtained from the cattle (No: 7) and 82.6% (No: 19) of sheep isolates were infected with F. hepatica species, whereas 17.4% (No: 4) of sheep isolates were infected with F. gigantica. It was also shown that F. hepatica was the predominant species of Fasciola present in the region. All the specimens were infected with Dicrocoelium dendriticum (D. dendriticum).
Both the species of Fasciola were found in Qazvin. D. dendriticum was the sole infecting species of the Dicrocoelium genus in the livestock of the city of Qazvin. Further research studies are needed to determine the intermediate host of the parasites in the region.
片形吸虫病和双腔吸虫病是伊朗不同地区最常见的人畜共患疾病，对人类健康问题的影响日益增加。该国存在两种片形吸虫，即肝片形吸虫（Fasciola hepatica）和巨片形吸虫（Fasciola gigantica）。分子方法在鉴定这两个物种方面具有决定性作用。本研究旨在通过扩增 ITS-2 和 28S rDNA 基因序列来检测片形科和双腔科。
总共从伊朗卡泽伦的牲畜中采集了 30 个受感染的肝脏样本。从不同的牲畜中收集成虫。进行核糖体 RNA 基因区（ITS2）和 28S rDNA 基因片段的 DNA 提取和 PCR 扩增，并构建系统发育树。
从牛（No：7）和 82.6%（No：19）的绵羊分离物中获得的所有分离物均感染了肝片形吸虫（Fasciola hepatica），而 17.4%（No：4）的绵羊分离物感染了巨片形吸虫（Fasciola gigantica）。还表明，肝片形吸虫是该地区存在的主要片形吸虫种类。所有标本均感染了树枝状双腔吸虫（Dicrocoelium dendriticum）。
在卡泽伦发现了两种片形吸虫。在卡泽伦市的牲畜中，树枝状双腔吸虫是双腔属唯一感染的物种。需要进一步的研究来确定该地区寄生虫的中间宿主。
