Lo Mame-Marietou, Benfodda Zohra, Dunyach-Rémy Catherine, Bénimélis David, Roulard Romain, Fontaine Jean-Xavier, Mathiron David, Quéro Anthony, Molinié Roland, Meffre Patrick
UNIV. NIMES, UPR CHROME, 5 rue du Dr Georges Salan, F-30021 Nîmes Cedex 1, France.
Institut National de la Santé et de la Recherche Médicale, U1047, Montpellier University, CHU de Nîmes, Place du Pr R. Debré, 30029 Nîmes, France.
ACS Omega. 2022 Sep 27;7(40):35851-35862. doi: 10.1021/acsomega.2c04195. eCollection 2022 Oct 11.
Plants are an everlasting inspiration source of biologically active compounds. Among these medicinal plants, the biological activity of extracts from some species of the genus has been studied, but the phytochemistry of the hardy species remains unknown. The aim of the present study was to perform the first phytochemical study of and to identify the compounds responsible for the antibacterial activity of extracts. Soxhlet extraction of predried and grinded leaves was first performed using four increasing polarity solvents. A bio-guided fractionation was performed using agar overlay bioautography as a screening method against 12 Gram-positive, Gram-negative, sensitive, and resistant bacterial strains. The results showed the inhibition of Gram-positive methicillin-sensitive ATCC 29213 (MSSA), methicillin-resistant N-SARM-1 (MRSA), and ATCC 35538 by the dichloromethane fraction. A phytochemical investigation led to the isolation and identification by high-resolution mass spectrometry and nuclear magnetic resonance of the two flavones penduletin and viscosine, responsible for this antibacterial activity. For viscosine, the minimum inhibitory concentration (MIC) value is equal to 128 μg/mL against MSSA and is equal to 256 μg/mL against MRSA and . The combination of these compounds with vancomycin and cloxacillin showed a decrease in MICs of the antibiotics. Penduletin showed synergistic activity when combined with vancomycin against MSSA (FICI < 0.258) and (FICI < 0.5). Thus, unexplored species may represent a valuable source for potential antibiotics and adjuvants.
植物是生物活性化合物的永恒灵感来源。在这些药用植物中，已经对该属某些物种提取物的生物活性进行了研究，但该耐寒物种的植物化学性质仍然未知。本研究的目的是对[植物名称]进行首次植物化学研究，并鉴定负责[植物名称]提取物抗菌活性的化合物。首先使用四种极性递增的溶剂对预干燥并研磨的叶子进行索氏提取。使用琼脂覆盖生物自显影作为针对12种革兰氏阳性、革兰氏阴性、敏感和耐药细菌菌株的筛选方法进行生物导向分级分离。结果表明，二氯甲烷馏分对革兰氏阳性甲氧西林敏感的金黄色葡萄球菌ATCC 29213（MSSA）、耐甲氧西林的金黄色葡萄球菌N-SARM-1（MRSA）和金黄色葡萄球菌ATCC 35538有抑制作用。通过高分辨率质谱和核磁共振进行的植物化学研究导致分离并鉴定出两种黄酮penduletin和viscosine，它们负责这种抗菌活性。对于viscosine，对MSSA的最低抑菌浓度（MIC）值等于128μg/mL，对MRSA和[金黄色葡萄球菌ATCC 35538]等于256μg/mL。这些化合物与万古霉素和氯唑西林的组合显示抗生素的MIC值降低。Penduletin与万古霉素联合使用时对MSSA（FICI < 0.258）和[金黄色葡萄球菌ATCC 35538]（FICI < 0.5）表现出协同活性。因此，未被探索的[植物名称]物种可能是潜在抗生素和佐剂的宝贵来源。
