Kemal Teyiba, Feyisa Kebede, Bisrat Daniel, Asres Kaleab
Department of Pharmacy, College of Health and Medical Science, Haramaya University, Harar, Ethiopia.
Department of Pharmaceutical Chemistry and Pharmacognosy, School of Pharmacy, College of Health Sciences, Addis Ababa University, Addis Ababa, Ethiopia.
J Trop Med. 2022 Oct 7;2022:3391216. doi: 10.1155/2022/3391216. eCollection 2022.
The leaves of Salzm. ex Decne, endemic to Ethiopia, are traditionally used for the treatment of malaria. Previous phytochemical investigations of species showed the presence of flavonoids, anthracene derivatives, and sesquiterpene lactones as the main constituents. The aim of the present study was to investigate the antimalarial activity of the leaf extract of and its isolated constituent against mice infected with .
Isolation of a compound was carried out on silica gel column chromatography of the extract eluting with gradient mixtures of CHCl/MeOH. Structural elucidation of the isolated compound was achieved by ESI-MS and 1D-and 2D-NMR spectral data. Peter's 4-day suppressive test method was used to determine the antimalarial activity of the test substances. Level of parasitemia, survival time, and body weight change were used to determine the antimalarial activity of the test substances.
(-) Catechin was isolated and characterized from the hydroalcoholic extract of . At a concentration of 400 mg/kg, both the extract and (-) catechin exhibited antimalarial activity with the highest chemosuppression values of 70.61% and 64.26%, respectively.
These findings indicate that is endowed with genuine antimalarial activity attributed in part, to its (-) catechin content. Hence, the present study may validate the traditional use of the plant for the treatment of malaria.
Salzm. ex Decne的叶子原产于埃塞俄比亚，传统上用于治疗疟疾。先前对该物种的植物化学研究表明，其主要成分包括黄酮类化合物、蒽衍生物和倍半萜内酯。本研究的目的是研究该植物叶提取物及其分离成分对感染疟原虫的小鼠的抗疟活性。
通过用CHCl/MeOH梯度混合物洗脱提取物，在硅胶柱色谱上进行化合物的分离。通过ESI-MS和一维及二维NMR光谱数据对分离出的化合物进行结构解析。采用彼得4天抑制试验方法测定受试物质的抗疟活性。用疟原虫血症水平、存活时间和体重变化来测定受试物质的抗疟活性。
从该植物的水醇提取物中分离并鉴定出（-）儿茶素。在浓度为400mg/kg时，提取物和（-）儿茶素均表现出抗疟活性，化学抑制率最高分别为70.61%和64.26%。
这些发现表明该植物具有真正的抗疟活性，部分归因于其（-）儿茶素含量。因此，本研究可能验证了该植物治疗疟疾的传统用途。
