Teferi Meseret Yigzaw, Abdulwuhab Mohammedbirhan, Yesuf Jibril Seid
1 Debre Tabor Hospital, Debre Tabor, Ethiopia.
2 University of Gondar, Gondar, Ethiopia.
J Evid Based Integr Med. 2019 Jan-Dec;24:2515690X19833340. doi: 10.1177/2515690X19833340.
The leaf of Osyris quadripartita is traditionally used for the management of diarrhea in different parts of Ethiopia. However, its use has not been scientifically validated for its efficacy. The aim of this study was to investigate antidiarrheal activity of hydroalcoholic leaf extract of O. quadripartita in mice models. Different doses of the methanolic leaf extract of O. quadripartita (100, 200, and 400 mg/kg) were tested for antidiarrheal activity using castor oil-induced diarrhea, enteropooling, and gastrointestinal motility models in Swiss Albino mice. The activities of the extract at different doses were compared with standard drugs and negative control groups of mice. The extract at all tested doses resulted in significant reduction ( P < .01) in number of wet feces, whereas significant reduction ( P < .01) in frequency of defecation in castor oil-induced diarrhea was seen at a dose of 400 mg/kg. It also showed a dose-dependent and significant reduction of volume of intestinal content in the enteropooling model at all tested doses and the observed results in 200 and 400 mg/kg were better than the standard drug, loperamide. However, significant antimotility effect was not observed at any of the tested doses. From these results we can conclude that methanolic leaf extract of O. quadripartita showed antidiarrheal activity.
在埃塞俄比亚的不同地区，四方沙针的叶子传统上用于治疗腹泻。然而，其疗效尚未得到科学验证。本研究的目的是在小鼠模型中研究四方沙针水醇叶提取物的止泻活性。使用蓖麻油诱导的腹泻、肠积液和胃肠动力模型，对不同剂量的四方沙针甲醇叶提取物（100、200和400mg/kg）进行止泻活性测试。将不同剂量提取物的活性与标准药物组和小鼠阴性对照组进行比较。所有测试剂量的提取物均导致湿粪数量显著减少（P<.01），而在400mg/kg剂量下，蓖麻油诱导的腹泻中排便频率显著降低（P<.01）。在肠积液模型中，所有测试剂量的提取物均显示出剂量依赖性且显著减少肠内容物体积，并且在200和400mg/kg剂量下观察到的结果优于标准药物洛哌丁胺。然而，在任何测试剂量下均未观察到显著的抗动力作用。从这些结果我们可以得出结论，四方沙针甲醇叶提取物具有止泻活性。
