Falck J R, Schueler V J, Jacobson H R, Siddhanta A K, Pramanik B, Capdevila J
J Lipid Res. 1987 Jul;28(7):840-6.
Epoxyeicosatrienoic acids were isolated and purified from female rabbit kidneys. They were identified as a group, prior to resolution, by packed column gas-liquid chromatography-mass spectroscopic techniques as their methyl esters as well as their trimethylsilyl bromohydrin methyl esters. Initial capillary gas-liquid chromatography-mass spectral analysis of the corresponding hydrogenated pentafluorobenzyl esters revealed the presence of the 8,9- and 14,15-epoxyeicosatrienoate regioisomers. These results, in conjunction with the documented in vitro biological activities of the arachidonate epoxygenase metabolites, suggest a role for them in renal function.
从雌性兔肾中分离并纯化出环氧二十碳三烯酸。在分离之前，通过填充柱气液色谱 - 质谱技术将它们作为甲酯以及三甲基甲硅烷基溴代醇甲酯进行了整体鉴定。对相应的氢化五氟苄基酯进行的初步毛细管气液色谱 - 质谱分析显示存在8,9 - 和14,15 - 环氧二十碳三烯酸酯区域异构体。这些结果，结合已记录的花生四烯酸环氧化酶代谢产物的体外生物活性，表明它们在肾功能中发挥作用。
