Division of Radiation Biomedical Research, Korea Institute of Radiological and Medical Sciences, Seoul 01812, Republic of Korea.
Department of Life Sciences, Korea University, 145 Anam-ro Seongbuk-gu, Seoul 02841, Republic of Korea.
J Radiat Res. 2022 Dec 6;63(6):817-827. doi: 10.1093/jrr/rrac061.
The primary motivation of this investigative study is trying to find an alternative treatment that can be used to slow down or treat glioblastoma due to the witnessed toxic side effects of the current drugs coupled with limited effectiveness in overall treatment. Consequently, a Chinese plant extract emodin proves to play a critical role in this investigative study since results from the Western blot and the other accompanying assays for anti-cancer effects indicate that it cannot work a lot to suppress cell migration and possible invasion, but rather emodin can be combined with radiation to give desired outcomes. Our result shows that the kind of radiation which acts well with emodin is neutron radiation rather than gamma radiation. Emodin significantly enhanced the radiosensitivity of LN18 and LN428 cells to γ-rays through MTT assay and cell counting. Accordingly, exposure to neutron radiation in the presence of emodin induced apoptotic cell death and autophagic cell death to a significantly higher extent, and suppressed cell migration and invasiveness more robustly. These effects are presumably due to the ability of emodin to amplify the effective dose from neutron radiation more efficiently. Thus, the study below is one such trial towards new interventional discovery and development in relation to glioblastoma treatment.
本研究的主要动机是试图寻找一种替代疗法，以减缓或治疗胶质母细胞瘤，因为目前的药物存在毒性副作用，而且整体治疗效果有限。因此，一种中国植物提取物大黄素在本研究中起着关键作用，因为来自 Western blot 和其他伴随的抗癌作用的结果表明，它不能有效地抑制细胞迁移和可能的侵袭，而是可以与辐射结合以达到预期的效果。我们的结果表明，与大黄素协同作用的辐射类型是中子辐射而不是伽马辐射。通过 MTT 检测和细胞计数，大黄素显著增强了 LN18 和 LN428 细胞对γ射线的放射敏感性。因此，在大黄素存在的情况下，中子辐射诱导细胞凋亡和自噬性细胞死亡的程度显著增加，并且更有效地抑制细胞迁移和侵袭。这些作用可能是由于大黄素能够更有效地放大来自中子辐射的有效剂量。因此，下面的研究是针对胶质母细胞瘤治疗的新干预发现和发展的一项尝试。
